聚乙基植入物的表面毛孔结构调节机械性质,促进巨细胞M2极化
Yixuan Chen1, Sida Liu1,2, Gandong Zhou3
1Department of Ophthalmology, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710038, China.
ACS applied materials & interfaces
|October 2, 2025
概括
聚乙基 (PEEK) 植入物上的统一的200纳米多孔结构促进巨细胞M2极化,通过Piezo1/TGF-β1通路增强软组织的整合.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 免疫学 免疫学 免疫学
背景情况:
- 聚乙 (PEEK) 上的表面微纳米结构可以提高生物相容性和软组织融合.
- 控制PEEK表面纳米结构以实现可预测的巨细胞M2极化是具有挑战性的.
- 了解PEEK表面的巨细胞M2极化机制至关重要.
研究的目的:
- 在PEEK上使用热压制制造均的亚微米孔状结构.
- 为了研究诱导巨细胞M2偏振的最佳孔径.
- 阐明PEEK介导的巨细胞M2极化和软组织集成背后的分子机制.
主要方法:
- 热压技术以创建具有200,500和800nm孔的PEEK表面.
- 在体外细胞实验中评估巨细胞M2极化和细胞因子分泌 (TGF-β1,Arg1).
- 使用内皮细胞和纤维细胞进行间接培试验,转录组分析,以及在生体内小鼠植入研究.
主要成果:
- 200nm多孔PEEK显著增加了水友性和降低了刚性.
- 最佳的200nm毛孔促进了巨细胞M2极化,增加了TGF-β1和Arg1的分泌.
- 极化巨细胞增强了血管内皮细胞和纤维细胞的增殖和迁移.
- 200nmPEEK降低了Piezo1,Yap1和NF-κB的调节,同时提高了STAT6和TGF-β1.1的调节.
- 在体内研究表明,在200nmPEEK植入物周围改善了软组织整合,血管化和纤维化.
结论:
- 200纳米的多孔PEEK表面有效调节巨细胞M2极化,以增强软组织的整合.
- Piezo1/TGF-β1信号通路对于PEEK诱导的巨细胞M2极化至关重要.
- 定制PEEK表面纳米结构为改善植入物生物相容性和骨融合提供了一个有希望的策略.
更多相关视频
07:55A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces
Published on: January 7, 2020
7.8K
08:37Polarization and Characterization of M1 and M2 Human Monocyte-Derived Macrophages on Implant Surfaces
Published on: December 6, 2024
1.7K
相关概念视频
Assembly of Complex Microtubule Structures
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
Cell Motility through Blebbing
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
