基于益生菌的衍生型外聚糖-氧化纳米颗粒:一种新的方法来调节结直肠癌基因
Saeideh Afsharipoor1, Razieh Nazari1, Anoosh Eghdami2
1Department of Microbiology, Qo.C, Islamic Azad University, Qom, Iran.
Bioorganic chemistry
|October 2, 2025
概括
这项研究探讨了益生菌外聚糖 (EPS) 和氧化纳米颗粒 (ZnONPs) 如何影响结肠直肠癌 (CRC) 细胞. 研究结果表明,这些药物可以调节关键基因,为CRC治疗提供潜在的新策略.
科学领域:
- 生物技术是生物技术.
- 纳米医学是一种纳米医学.
- 分子生物学分子生物学
背景情况:
- 结肠直肠癌 (CRC) 是全球癌症死亡的主要原因,其特点是无法控制的结肠上皮细胞增殖.
- 益生菌及其外聚糖 (EPS) 成分表现出抗氧化和抗癌特性,表明其治疗潜力.
- 氧化纳米颗粒 (ZnONPs) 具有适用于癌症诊断和治疗的独特特性.
研究的目的:
- 为了研究氧化纳米颗粒 (ZnONPs) 的生物合成,使用来自Lactiplantibacillus plantarum的外聚糖 (EPS).
- 评估EPS和生物合成ZnONPs在SW480结直肠癌细胞系上的细胞毒性和抗氧化作用.
- 分析EPS和ZnONPs对关键基因 (ING4,APC,DHX32,P53) 参与结直肠癌进展的表达的影响.
主要方法:
- 用EPS从Lactiplantibacillus plantarum中提取出来的ZnONPs的生物合成.
- 使用UV-VIS,XRD,FTIR,FE-SEM和泽塔潜力分析对生物合成的ZnONPs进行表征.
- 评估EPS和ZnONPs在SW480细胞上的细胞毒性和抗氧化活性.
- 定量实时PCR测定ING4,APC,DHX32和P53的基因表达水平.
主要成果:
- 通过EPS介导的ZnONPs生物合成成功实现并进行了表征.
- EPS和ZnONPs对SW480细胞表现出细胞毒性作用,并表现出抗氧化特性.
- 用EPS和ZnONPs治疗导致DHX32和P53的表达减少,并在SW480细胞中增加ING4和APC的表达.
结论:
- 益生菌衍生的EPS和生物合成的ZnONPs显示出作为结直肠癌的潜在治疗剂的希望.
- 这些药物有效调节参与CRC发育和进展的关键基因 (ING4,APC,DHX32,P53) 的表达.
- 这些发现表明,使用天然化合物和纳米技术进行CRC治疗的新战略.
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