聚烯纳米塑料通过增强分子间相互作用来调节VGLL3相分离:对纤维化和超越纤维化的影响
Mei Dang1, Qinqin Deng2, Longjiang Wu3
1School of Chemical and Environment Sciences, Shaanxi University of Technology, Hanzhong, Shaanxi 723000, China; College of Biological Sciences and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723000, China; School of Life Sciences, Westlake University, Hangzhou, Zhejiang 310030, China.
Journal of hazardous materials
|October 2, 2025
概括
富含碳酸盐的纳米颗粒诱导了纤维化调节剂VGLL3中的蛋白质液液相分离 (LLPS). 这揭示了微塑料和纳米塑料 (MNP) 如何影响纤维化,为MNP健康风险提供了洞察力.
科学领域:
- 环境科学 环境科学
- 生物化学 生化学
- 材料科学 材料科学 材料科学
背景情况:
- 微塑料和纳米塑料 (MNP) 是具有潜在健康影响的普遍污染物.
- 对蛋白质液液相分离 (LLPS) 的MNP的影响,这对细胞调节至关重要,并与纤维化有关,在很大程度上是未知的.
- 遗质类家族成员3 (VGLL3) 是一种转录辅因子,参与纤维化.
研究的目的:
- 研究不同表面化学和大小的聚乙烯纳米颗粒 (PSNP) 对VGLL3.3相态行为的影响.
- 阐明MNP可能影响纤维性疾病相关的LLPS蛋白的机制.
主要方法:
- 通过PS NPs与炭基 (-COOH),氨基 (-NH2) 和裸体表面使用不同度和大小,研究了VGLL3 LLPS诱导.
- 描述了使用1,6-hexanediol的VGLL3凝结物的动态和可逆性质.
- 利用动态光散射 (DLS) 来分析NP-蛋白质复合体的形成.
- 采用分子对接来模拟PS-COOHNP和VGLL3之间的相互作用.
主要成果:
- 强大而选择性诱导VGLL3LLPS的PS-COOHNP以一种依赖于度和大小的方式.
- 在较高的PS-COOH NP分数下观察到VGLL3凝析物的回流溶解.
- 在VGLL3LLPS上,PS NP和PS-NH2 NP对VGLL3LLPS的影响很小.
- 分子对接提出了一个支架模型,其中PS-COOH NP表面调解VGLL3多价值相互作用.
结论:
- 富含碳酸盐的MNP可以调节VGLL3的相位行为,VGLL3是纤维化中的关键调节器.
- 这项研究提供了一个机械的理解,具体的MNP表面化学如何可以改变蛋白质凝结物.
- 结果为评估MNP相关的健康风险和设计更安全的纳米材料提供了一个框架.
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