相关实验视频
Updated: Jan 16, 2026

10:23
Analysis of Epididymal Protein Synthesis and Secretion
Published on: August 25, 2018
9.8K
精子细胞外囊泡通过调节关键功能参数来影响猪精子生理学
Ana Parra1, Francisco E Martín-Cano2, Pablo Martínez-Díaz1
1Department of Medicine and Animal Surgery, Veterinary Science, University of Murcia, Murcia, Spain; International Excellence Campus for Higher Education and Research "Campus Mare Nostrum", Institute for Biomedical Research of Murcia (IMIB‑Arrixaca), University of Murcia, Murcia, Spain.
Animal reproduction science
|October 2, 2025
概括
精子细胞外囊泡 (sEVs) 与猪精子相互作用,减少氧化应激和线粒体活动,同时增加亡. 这些发现澄清了sEV在各种pH条件下的精子功能中的作用.
科学领域:
- 生殖生物学 生殖生物学
- 细胞外囊泡研究研究
- 精子生理学 精子生理学
背景情况:
- 精液等离子体 (SP) 含有细胞外囊泡 (EVs),调节精子功能.
- 不同种子EV (sEV) 子集的具体作用尚不清楚.
- 研究sEV与精子之间的相互作用对于理解生殖过程至关重要.
研究的目的:
- 为了评估小 (S-sEVs) 和大 (L-sEVs) 子集与猪精子的相互作用.
- 为了确定这些sEV子集在不同pH条件下对精子功能的影响.
- 阐明sEVs对氧化应激,线粒体潜力和精子细胞的亡的影响.
主要方法:
- 从精液等离子体中分离和描述sEV子集,使用大小排除色谱.
- 在不同的pH条件下 (6.5,7.0,7.5) 用sEV子集化猪精液.
- 评估精子的功能参数,包括活性氧物种,线粒体膜潜力和通过流动细胞计和共聚焦显微镜的亡.
主要成果:
- 无论pH值如何,S-sEV和L-sEV都容易与精子结合并被精子内部化.
- sEVs显著降低了活性氧物种的产生和线粒体活动.
- sEVs增加了活性的精子细胞的亡,对宏细胞完整性的影响取决于时间和条件.
结论:
- 小型和大型精子EV与生理pH范围内的猪精子相互作用.
- 这种相互作用导致精子中氧化应激和线粒体活性减少.
- 这项研究强调了sEVs在调节精子生存能力和功能的复杂作用,包括增加了亡.
相关概念视频
Sperm Structure and Semen Composition
10.4K
During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...
10.4K
Spermatogenesis
121.9K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
121.9K
Sperm Transport
3.1K
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
The maturation phase occurs in the epididymis, where sperm...
3.1K
Overview of Exosomes
3.5K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
3.5K

