消化污水污泥的机床级固态发酵,以产生Bacillus thuringiensis:消化特性的影响
J Font-Pomarol1, J Kaal2, E Molina-Peñate1
1GICOM Research Group, Department of Chemical, Biological and Environmental Engineering, School of Engineering, Edifici Q, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, 08193, Spain.
Journal of environmental management
|October 2, 2025
概括
废水污泥可以作为使用固态发酵 (SSF) 生产生物农药的基质. 描述污泥特性是优化Bacillus thuringiensis生长和子生产为有价值的生物产品的关键.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
- 废弃物价值化 废弃物价值化 废弃物价值化
背景情况:
- 消化的污水污泥的固体分量含有丰富的有机物质和营养素.
- 污泥通常是土地应用,堆肥或处置,代表了一个错过的资源.
- 固态发酵 (SSF) 提供了一个将污泥转化为可销售的生物制品的途径,例如生物杀虫剂.
研究的目的:
- 描述不同的消化的污水污泥,以确定它们是否适合作为SSF的基板.
- 为了评估污泥特性对Bacillus thuringiensis生长和化的影响.
- 评估扩大生物农药生产的SSF工艺的可行性和可重复性.
主要方法:
- 综合描述四种不同的消化的污水泥,使用热解气体染色学-质谱学,物理化学分析和生物降解性测试.
- 实验室规模的SSF实验,以评估Bacillus thuringiensis在特征污泥上的生长和分泌.
- 使用选定的污泥进行基板规模扩大试验,以评估工艺的稳定性和子产量.
主要成果:
- 观察到胞产量的显著变化 (0.411.3胞/初始可活细胞),与固有的污泥特性有关.
- 突菌可以通过SSF在四个测试的污泥基板中的三个中成功生长.
- 扩大规模的试验表明,胞增加和可活细胞产量增加,达到2.3 × 10^8子/g干物质.
结论:
- 消化的污水污泥可以成为SSF生产生物杀虫剂Bacillus thuringiensis子的可行基质.
- 先进的污泥表征对于优化SSF性能和确保可靠的生物产品产量至关重要.
- 这种利用途径与循环经济原则保持一致,通过将废物转化为有价值的资源.
相关概念视频
01:29Microbes in Food Production
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
01:27Microbes in the Production of Fermented Foods
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
01:29Bioreactor Design and Operational System
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
01:14Scale-Up Processes
The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
01:25Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
01:18Production of Biopesticides
Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...