bZIP转录因子BcbZIP72通过CBF依赖的途径在非标题中国白菜中积极调节耐寒性
Guangpeng Wang1, Xinfeng Xu1, Hongjie Lu1
1National Key Laboratory of Crop Genetics & Germplasm Innovation and Utilization, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture and Rural Affairs of China, Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Ministry of Education of China, Nanjing Agricultural University, Nanjing, 210095, China.
Plant physiology and biochemistry : PPB
|October 2, 2025
概括
一个关键的基因,BcbZIP72,通过激活CBF基因,增强了中国白菜的耐寒性. 这一发现为培育抗寒应激的作物提供了基础.
科学领域:
- 植物科学 植物科学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 寒冷压力严重影响非标题中国白菜 (NHCC) 的生长和产量.
- 在NHCC冷反应中,G组基本氨酸拉链 (bZIP) 转录因子在NHCC冷反应中的作用尚不清楚.
研究的目的:
- 为了研究BcbZIP72的功能,一个G组bZIP基因,在NHCC冷应激反应中.
- 阐明BcbZIP72介导的寒冷耐受性背后的分子机制.
主要方法:
- 在NHCC中识别和描述BCcbZIP基因.
- 亚细胞局部化,转录激活检测和GUS染色.
- 在NHCC和Arabidopsis中进行基因表达分析 (过度表达和VIGS介导的沉默).
- 促进体分析以确定BcbZIP72的结合部位.
主要成果:
- 确定了150个BcbZIP基因; BcbZIP72表现出强烈的感冒诱导的表达.
- BcbZIP72是一种核局部转录激活剂.
- 过度表达BcbZIP72通过调节CBF和COR基因来增强耐寒性.
- BcbZIP72直接与BcCBF1促进体结合,作为一个上游调节器.
- 冷诱导的BcbZIP72表达是ABA独立的.
结论:
- BcbZIP72是NHCC中通过CBF依赖途径的寒冷耐受性的关键调节者.
- 这项研究为开发耐寒NHCC品种提供了理论基础.
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