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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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相关实验视频

Updated: Jan 16, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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在单分子分辨率下无测序全基因组空间转录组学

Yubao Cheng1, Shengyuan Dang1, Yuan Zhang1

  • 1Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA.

Cell
|October 2, 2025
PubMed
概括

研究人员开发了RAEFISH,一种新的空间转录组学方法,提供全基因组覆盖和单个分子分辨率. 这项技术能够在完整的组织中绘制高分辨率的基因表达图,从而推进细胞和组织分析.

关键词:
高含量的CRISPR屏幕高多重化RNA成像空间转录学

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科学领域:

  • 分子生物学
  • 基因组学
  • 生物技术

背景情况:

  • 空间转录学促进了细胞和组织的理解.
  • 目前的方法在转录覆盖和空间分辨率方面存在局限性.
  • 没有偏见,高分辨率的转录组分析仍然是一个挑战.

研究的目的:

  • 开发一种具有增强覆盖和分辨率的新型空间转录组学方法.
  • 在完整的组织中实现无偏见,无假设的转录组分析.
  • 在整个基因组中以单分子分辨率对基因表达进行分析.

主要方法:

  • 开发了反向锁安普利康编码的现场混合体 (RAEFISH).
  • RAEFISH是一种基于图像的空间转录学技术.
  • 将RAEFISH应用于完整的人类和小鼠组织以进行转录分析.

主要成果:

  • 在现场实现全基因组覆盖和单分子分辨率.
  • 证明了大约23,000个人类或大约22,000个小鼠基因的空间概况.
  • 揭示了转录本地化,细胞类型特定的转录组和细胞相互作用的基因程序.
  • 在CRISPR屏幕中适应RAEFISH用于导向RNA的空间读取.

结论:

  • RAEFISH提供了广泛适用的高覆盖率,高分辨率的空间RNA分析技术.
  • 能够在多种生物医学环境中对原生和人工RNA进行详细分析.
  • 促进了解细胞功能,组织和相互作用的进步.