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Zhang Qing¹, Huang Haibei², Yin Hanxue²

¹Shandong Key Laboratory for Germplasm Innovation of Saline-alkaline Tolerant Grasses and Trees, College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, China; College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.

Bioresource technology

|October 2, 2025

概括

乳酸细菌 (LAB) 接种剂通过修改微生物组,有效地降低了灌中的四环素耐药基因 (TRGs). 这种有针对性的方法提供了一个管理化生态系统中抗菌素耐药性的策略.

科学领域:

微生物学微生物学
环境科学环境科学
遗传学是一个遗传学.

背景情况:

灌生态系统中的抗生素耐药性基因 (ARG) 威胁着生态稳定和公共健康.
抗四环素基因 (TRG) 是料中最常见的ARG.
乳酸细菌 (LAB) 是料发酵中的关键参与者.

研究的目的:

调查特定的LAB接种剂对料中的TRG的影响.
评估LAB在化物管理中的抗菌素耐药性减轻方面的潜力.

主要方法:

根据生长和酸化,分离和选择了四种LAB菌株 (Lactiplantibacillus plantarum,Lacticaseibacillus paracasei).
将LAB接种剂应用于化,并分析发酵参数 (pH,氨-N,大肠杆菌).
基因组测序和定量PCR用于评估ARG配置文件和特定TRG (tetA,tetG) 的变化.

主要成果:

选择的LAB菌株显著降低了化物pH,氨-N和大肠杆菌数量.
在 LAB 疫苗接种过程中,促进了 Lactiplantibacillus 和 Lacticaseibacillus 种类的主导地位.
化过程和LAB接种导致了各种ARG的显著抑制,包括特定的TRG,如tetA和tetG.
特定的TRG显示与Lactiplantibacillus和Lacticaseibacillus物种的丰富度存在相关性.

结论:

发酵过程显著改变了料中的ARG概况.
以LAB为媒介的微生物组工程是一种可行的策略,用于抑制料中的TRGs.
这项研究为精密灌管理提供了基础,以减轻抗菌素耐药性.

关键词:

抗生素耐药性基因是抗生素耐药性的基因.发酵质量的质量微生物社区的微生物社区.定量PCR是一种定量PCR.

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