在巨细胞内聚烯纳米塑料的溶解体依赖转细胞化
Zijia Liu1, Yunjiang Yu2, Yuchen Zheng1
1College of Chemistry & Life Science, Beijing University of Technology, Beijing, 100124, China.
概括
聚乙烯纳米塑料 (PS-NP) 进入人体细胞并触发 lysosomal exocytosis,通过依赖的途径释放蛋白质 cathepsin D (CTSD). 这项研究揭示了纳米塑料细胞运输的新机制.
科学领域:
- 环境科学 环境科学
- 细胞生物学 细胞生物学
- 毒理学 毒理学 毒理学
背景情况:
- 纳米塑料 (NP) 是新兴的环境污染物,具有潜在的健康风险.
- 有限的研究存在于NP的细胞内迁移和外细胞分裂.
研究的目的:
- 为了研究人类巨细胞中聚烯纳米塑料 (PS-NP) 的细胞内命运.
- 阐明PS-NP细胞外的机制,并确定相关的细胞通路.
主要方法:
- 人类巨细胞暴露于PS-NPs.
- 蛋白质和生物信息分析以确定蛋白质吸附.
- 测量离子 (Ca2+),以确认路径中介.
主要成果:
- 在溶解体中积累的PS-NP引发了溶解体激活并增加了溶解体含量.
- PS-NPs吸附了参与 lysosomal 途径的蛋白质,尤其是 cathepsin D (CTSD).
- PS-NPs诱导了溶酶体外细胞分裂,同时以Ca2+依赖的方式释放成熟的CTSD.
结论:
- 这项研究阐明了PS-NPs的溶酶体依赖转细胞路径.
- 在PS-NPs上吸附的成熟CTSD作为验证 lysosomal exocytosis的新标志物.
关键词:
lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis lysosomal exocytosis 是一种发生在细胞内发生的细胞体内发生的过程中发生的巨细胞是一个巨细胞.纳米塑料是一种纳米塑料.蛋白质吸附是一种蛋白质吸附.
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