动物起源的Clostridium perfringens的基因组测序和比较基因组分析
Kumaragurubaran Karthik1, Subbaiyan Anbazhagan2, Prasad Thomas3
1Veterinary College and Research Institute, Udumalpet, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India.
Microbial pathogenesis
|October 2, 2025
概括
克洛斯特里迪亚菌株表现出显著的遗传多样性和适应性进化. 基因组分析揭示了广泛的抗微生物耐药性和潜在的动物传染风险,强调了需要"一个健康"监测的必要性.
科学领域:
- 兽医微生物学 兽医微生物学
- 基因组学就是基因组学.
- 一个健康研究研究.
背景情况:
- 杆菌 (Clostridium perfringens) 是一种引起人类和动物肠道疾病的动物性细菌.
- 抗微生物耐药性 (AMR) 和强毒素的产生是关键问题.
- 了解C. perfringens的遗传多样性对于疾病控制至关重要.
研究的目的:
- 执行全基因组测序和对来自印度和全球的C. perfringens分离物进行比较基因组分析.
- 为了研究遗传多样性,毒性因素和AMR模式.
- 评估C. perfringens在动物种群中的健康影响.
主要方法:
- 四个印度分离物和133个全球动物菌株的全基因组测序 (WGS).
- 遗传学分析,多部位序列类型 (MLST) 和全基因组多部位序列类型 (wgMLST).
- 泛基因组分析,毒性基因分析和抗菌素耐药性基因分析.
主要成果:
- 遗传学分析揭示了六个主要集群,没有明显的地理或宿主相关性.
- 两种印度分离物被确定为已知的序列类型 (ST126,ST200),两种是新鲜的.
- 具有高辅助基因含量的开放性泛体结构.
- 病毒性基因 (plc,pfoA,cpe) 是普遍存在的,特别是在单独的狗中.
- 抗四环素耐药性是最常见的;观察到多种药物耐药性.
- 毒素型F从聚集在一起的狗中分离出来,表明潜在的动物感染风险.
结论:
- 在动物宿主中,C. perfringens表现出显著的遗传多样性和适应性进化.
- 基因组洞察对于了解宿主特异性毒性和耐药性至关重要.
- 这些发现凸显了"一带一路"卫生方法对C. perfringens的监测和控制的重要性.
相关概念视频
01:29Modern Molecular Taxonomy
590
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
590
02:54Evolutionary Relationships through Genome Comparisons
6.9K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
6.9K
03:36Genome Annotation and Assembly
20.5K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
20.5K
02:02Genomics
39.6K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
39.6K