相关实验视频

Updated: Jan 16, 2026

An Inexpensive, Scalable Behavioral Assay for Measuring Ethanol Sedation Sensitivity and Rapid Tolerance in Drosophila

An Inexpensive, Scalable Behavioral Assay for Measuring Ethanol Sedation Sensitivity and Rapid Tolerance in Drosophila

Published on: April 15, 2015

miR-148a通过激活ADH4增强元件来促进多个酒精脱酶基因的表达

Zhe Huang¹, Shuyue Zhou¹, Jing Wang²

¹School of Public Health, Qingdao University, Qingdao, China.

International journal of biological macromolecules

|October 2, 2025

概括

微RNA-148a通过促进酒精脱酶 (ADH) 基因表达来增强酒精代谢. 这种微RNA通过增强元件激活ADH基因,影响肝损伤和酒精代谢调节.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
生物化学生物化学

背景情况:

酒精脱酶 (ADHs) 是酒精代谢中的关键酶,主要在肝脏中.
位于4号染色体上的ADH基因家族对酒精代谢和肝损伤至关重要.
控制ADH基因表达的调控机制尚不清楚.

研究的目的:

研究微RNA-148a (miR-148a) 在调节酒精脱酶 (ADH) 基因表达中的作用.
阐明miR-148a影响ADH基因表达的机制.
了解miR-148a对酒精诱导的肝细胞损伤的影响.

主要方法:

在人类肝脏组织中对miR-148a水平和ADH基因表达的相关性分析.
在正常条件下和暴露于乙醇的条件下评估miR-148a对ADH基因表达的影响的功能测试.
路西法酶记者测定以确定miR-148a介导增强剂激活的机制.

主要成果:

在miR-148a水平和ADH1A,ADH1B,ADH4和ADH6基因的表达之间发现了正相关性.
miR-148a促进了这些ADH基因的表达.
miR-148a通过准特定的动机,激活了ADH4基因内的增强元件.
miR-148a在肝细胞中加剧了乙醇诱导的细胞毒性.

结论:

miR-148a是一种酒精脱酶基因表达的新型调节剂.
miR-148a通过增强器激活来协调多个ADH基因的表达.
这一发现为酒精代谢的转录调节及其在肝损伤中的作用提供了新的见解.

关键词:

在ADH4中,ADH4为ADH4.酒精脱水酶的使用增强器增强器增强器乙醇乙醇是一种在 miR-148a 里面.

更多相关视频

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

Published on: January 23, 2019

Assessment of Glutamine as a Fuel Source for Alveolar Macrophages Exposed to Chronic Ethanol Using an Extracellular Flux Bioanalyzer

Assessment of Glutamine as a Fuel Source for Alveolar Macrophages Exposed to Chronic Ethanol Using an Extracellular Flux Bioanalyzer

Published on: November 15, 2024

相关概念视频

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

Exon Recombination

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...