在多药耐药结核病中的外围T细胞上的免疫检查点分子的免疫特征
Xiaoxu Yang1, Lan Yao2, Xu-Wei Gui2
1Shanghai Institute of Immunology, Department of Microbiology and Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
概括
耐多药结核病 (MDR-TB) 患者表现出增加的T细胞耗尽,特征是免疫检查点分子表达的增加. 治疗可以逆转这种疲劳,改善MDR-TB患者的T细胞免疫力.
科学领域:
- 免疫学 免疫学 免疫学
- 传染性疾病 传染性疾病
- 结核病研究 结核病研究
背景情况:
- 诸如结核病 (TB) 这样的慢性感染通过 T 细胞耗尽而损害 T 细胞免疫力.
- 多耐药结核病 (MDR-TB) 患者的特定免疫特征,特别是关于T细胞耗尽的特征,尚不清楚.
研究的目的:
- 与药物敏感结核病 (NR-TB) 患者相比,研究MDR-TB患者免疫检查点 (IC) 分子和T细胞增殖的表达.
- 为了确定MDR-TB中T细胞耗尽是否可以通过抗结核治疗逆转.
主要方法:
- 多重流细胞计用于分析来自MDR-TB (n=27) 和NR-TB (n=51) 患者的T细胞.
- 测量了IC分子 (CTLA-4,PD-1,TIM-3) 和增殖标志物Ki67的表达.
- 对MDR-TB患者的抗结核治疗前和期间分析了样本.
主要成果:
- 在治疗前,MDR-TB患者的T细胞表达CTLA-4,PD-1和TIM-3的百分比明显高于NR-TB患者.
- 在MDR-TB患者中观察到CTLA-4与PD-1和TIM-3的同时表达较高.
- 耐多药结核病患者表现出T细胞增殖受损,与增加的T细胞耗尽相关.
- 抗结核病治疗导致MDR-TB患者T细胞上IC分子表达的减少,接近NR-TB患者的水平.
结论:
- 多抗药性结核病患者的T细胞表现出更枯竭的表型,在成功治疗结核病后,这种表型是可逆的.
- 向T细胞耗尽可能提供一种新的治疗策略,以增强MDR-TB患者的抗结核免疫力.
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