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Updated: May 20, 2026

Robust 3D DNA FISH Using Directly Labeled Probes
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左撇子DNA以单蛋白分辨率高效的高复合成像

Eduard M Unterauer1,2, Eva-Maria Schentarra1,2, Isabelle Pachmayr2,3

  • 1Faculty of Physics and Center for Nanoscience, Ludwig Maximilian University, Munich, Germany.

Nature communications
|October 2, 2025
PubMed
概括

研究人员开发了一种使用DNA-PAINT用于空间蛋白质组学的更快的12倍位成像方法. 该技术实现了神经元蛋白相互作用的3D高分辨率映射,克服了以前方法的局限性.

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Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...