不同盐度下的维生素D补充对混合红色鱼生长性能,荷尔蒙特征和生长基因表达的影响
Hassan H Silem1, Amal M El-Nahla2, Ahmed G A Gewida3
1Aquatic Hatchery Production Department , Fish farming and Technology Institute, Suez Canal University , Ismailia, 41522, Egypt.
Scientific reports
|October 2, 2025
概括
这项研究表明,维生素D (VD) 和盐度可以增强红色鱼的生长. 最佳条件涉及30 ppt盐度与VD补充剂,改善生长,料使用和基因表达.
科学领域:
- 水产养殖和鱼类生理学
- 营养生物化学 营养生物化学
- 分子生物学分子生物学
背景情况:
- 红鱼杂交幼虫对环境因素的生理反应对于水产养殖的成功至关重要.
- 了解盐度和维生素D (VD) 之间的相互作用对于优化鱼类生长和健康至关重要.
- 之前的研究还没有完全阐明盐度和风病在红色鱼上在分子层面的联合影响.
研究的目的:
- 研究不同盐度水平和维生素D补充剂对红色鱼杂交幼虫的生长,激素特征和基因表达的影响.
- 确定最佳的盐度和VD水平,以提高红色鱼的生长性能和料利用率.
- 分析分子机制,包括生长激素 (GH) 和类似胰岛素的生长因子II (IGF-II) 基因表达,受盐度和静脉疾病的影响.
主要方法:
- 实验设计涉及不同的盐度处理 (0,15,30 ppt) 和维生素D3补充剂.
- 监测生长参数 (最终体重,平均每日增加,料转换率) 和生存率.
- 使用分子技术分析激素特征 (GH,ACTH) 和定量基因表达分析 (GH,IGF-II).
主要成果:
- 增长性能和存活率的显著改善在增加度和静脉疾病补充剂时观察到,度达到30 ppt,VD3.
- 在15-30 ppt盐度下,生长激素 (GH) 水平显著增加,而上腺皮质激素 (ACTH) 保持稳定,表明压力较低.
- 随着静脉疾病的出现,GH基因表达的升调在30 ppt盐度时被注意到,而IGF-II基因表达显示出复杂的反应,在0 ppt时增加,在30 ppt盐度下降.
结论:
- 盐度和维生素D补充剂协同增强红色鱼幼虫的生长,激素平衡和基因表达.
- 最佳的培养条件包括30 ppt盐度与维生素D补充剂 (0.8 mg,然后是0.4 mg) 结合,以改善生长和料效率.
- 与淡水培养相比,盐度的战略调整 (30 ppt 跟着 15 ppt) 可以优化红色鱼的表现,强调环境和营养管理的重要性.
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