在自发发酵的加纳谷物,豆类,根,根茎和大面粉中的细菌群体动态
Richard Atinpoore Atuna1, Fortune Akabanda2, Stefan Bletz3
1Department of Food Science and Technology, University for Development Studies, Tamale, Ghana. ratuna@uds.edu.gh.
BMC microbiology
|October 2, 2025
概括
这项研究探讨了加纳发酵食品中的细菌群落,揭示了由乳酸细菌主导的多样化的微生物生态系统. 了解这些动态可以提高发酵食品的安全性和质量.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
背景情况:
- 自发发酵是一种古老的食品保存技术,对营养和感官质量有好处.
- 在加纳的各种基质中,驱动这些转变的细菌群落尚不清楚.
- 迦纳土著的食物使用谷物,粉根,茎和植物.
研究的目的:
- 研究加纳选定的补充食品原料在自发发酵期间的细菌群体多样性.
- 在48小时发酵期间分析细菌群体与pH值和总可定位酸度 (TTA) 相关的动态.
- 为了确定参与玉米,小米,,甜,麻豆,大豆和大的发酵的关键细菌物种.
主要方法:
- 精选的加纳原材料 (玉米,小米,,甜,麻豆,大豆,大松) 被自发发酵48小时,每6小时采集样本.
- 在整个发酵过程中使用标准协议测量pH和TTA.
- 使用ZymoBIOMICSTM套件提取细菌DNA,然后在太平洋生物科学Sequel IIe平台上进行全长16SrDNA测序.
- 序列数据使用DADA2进行处理,并根据SILVA数据库 (v138.1) 进行分类,并通过R (v4.4.3) 进行分析.
主要成果:
- 发酵谷物显示出越来越多的细菌多样性,主要由Weissella confusa,Enterococcus hirae和Pediococcus acidilactici组成.
- 大豆发酵呈现出具有可变多样性的独特微生物群落.
- 富含粉的基板 (根,根茎,青菜) 支持了Leuconostoc mesenteroides,韦塞拉混,Gluconobacter frateurii,果菌菌果实菌和Pediococcus pentosaceus. 这三种植物的生长.
- 在酸性条件下壮成长的Enterococcus faecium,而Weissella confusa和Leuconostoc mesenteroides适应了波动的pH值.
结论:
- 经过时间的推移,发酵面粉呈现出增强的细菌多样性,以乳酸细菌 (LAB) 和酸细菌为主.
- 在不同的植物基质中观察到不同的发酵模式,受细菌行为和环境因素 (pH,TTA) 的影响.
- 了解这些细菌社区动态对于提高发酵食品的安全性,一致性和营养价值至关重要.
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