相关实验视频

Updated: Jan 16, 2026

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

用CRISPR编程的CuO纳米催化剂释放用于在无菌体液中超敏感检测病原体

Jiayi Xiao¹, Xiumei Hu², Hanren Chen¹

¹NMPA Key Laboratory for Research and Evaluation of Drug Metabolism & Guangdong Provincial Key Laboratory of New Drug Screening & Guangdong-Hongkong-Macao Joint Laboratory for New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China.

Analytical chemistry

|October 3, 2025

概括

一种新型的CRISPR相关蛋白12a (Cas12a) 编程纳米催化剂释放 (CNR) 方法可以快速,无培养地诊断无菌体液感染. 这种超敏感的测试在70分钟内检测到病原体,为资源有限的环境提供了一个有希望的解决方案.

科学领域:

生物技术是生物技术.
分子诊断学分子诊断学
纳米技术纳米技术

背景情况:

传统的无菌体液感染诊断需要长达72小时,延迟治疗.
与CRISPR相关的蛋白质12a (Cas12a) 技术为快速分子检测提供了潜力.
纳米催化剂系统可以在诊断试验中增强信号放大.

研究的目的:

开发一种快速的,无培养的诊断方法,用于使用Cas12a的无菌体液感染.
通过一种新的纳米催化剂释放策略来提高诊断灵敏度和特异性.
在临床样本中验证新方法的性能.

主要方法:

开发了一种CRISPR相关蛋白12a (Cas12a) 编程纳米催化剂释放 (CNR) 方法.
该试验使用初始DNA (sDNA) - 互补DNA (cDNA) 探针,准细菌/真菌rRNA.
激活的Cas12a将磁探头裂开,释放氧化铜纳米粒子 (CuONPs) 用于通过色度反应进行信号放大.

主要成果:

在原始无菌体液样本中,CNR方法实现了低至0.69 CFU mL-1的病原体检测.
该测试在70分钟内提供了结果,比传统方法快得多.
在临床样本中,该方法与培养相比显示出100%的灵敏度和100%的特异性,正确识别了培养负病例.

结论:

该CNR方法提供了一个快速的,超敏感的,无核酸放大的诊断解决方案.
这种方法特别适用于无菌体液感染和资源有限的环境.
这项技术显示出改善传染病诊断的巨大潜力.

更多相关视频

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

相关概念视频

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...