EPOP和MTF2通过DNA序列特异性激活PRC2活动
Jeffrey Granat1,2,3,4, Sanxiong Liu1,2,5,6,7,4, Luis Popoca1,2,8
1Howard Hughes Medical Institute, NYU Langone Health, New York, NY 10016, USA.
bioRxiv : the preprint server for biology
|October 3, 2025
概括
蛋白质EPOP增强了多合体抑制复合体2 (PRC2) 基因组甲基转移酶活性,有助于H3K27me3染色体域的形成. EPOP与MTF2和JARID2合作进行新的H3K27me3存储.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 染色体生物学 染色体生物学
- 分子生物学分子生物学
背景情况:
- 聚合体抑制复合体2 (PRC2) 建立了对组织特异性基因表达至关重要的可选性异质色素.
- PRC2催化了素H3氨酸27三甲基化 (H3K27me3),这是PRC1认可的染色质紧缩标记.
- 像MTF2和JARID2这样的辅因子引导PRC2到特定的DNA结合基因,调节其活性.
研究的目的:
- 研究EPOP在调节PRC2活性和染色体局部化中的作用.
- 为了阐明EPOP如何影响PRC2的基因组甲基转移酶 (HMT) 功能.
- 了解EPOP对H3K27me3沉积和染色体域形成的贡献.
主要方法:
- 在体外测试以测量PRC2 HMT活性与EPOP和MTF2.
- 在体内使用EED救援系统来评估PRC2染色质招募.
- 用EPOP和MTF2评估PRC2染色体结合活性的二核糖体结合试验.
主要成果:
- EPOP和MTF2都在体外刺激PRC2 HMT活动.
- 在活体中,EPOP并没有调解PRC2染色质的招募,而是促进了新的H3K27me3沉积与MTF2和JARID2.
- EPOP以DNA序列依赖的方式 (富含GCN) 增强PRC2染色体结合,类似于MTF2 (富含GA).
结论:
- EPOP作为一个PRC2相关的辅助因子,增强其HMT活动.
- EPOP,MTF2和JARID2合作促进H3K27me3在特定染色体位点的沉积.
- 这些共因子通过序列特定的DNA相互作用,共同促进H3K27me3-染色质域的形成.
相关概念视频
RNA Polymerase II Accessory Proteins
10.8K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
10.8K
Conservative Site-specific Recombination and Phase Variation
6.7K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
6.7K
Cooperative Binding of Transcription Regulators
7.2K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
7.2K
Prokaryotic Transcriptional Activators and Repressors
25.1K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
25.1K


