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对样本储存条件对外围动脉疾病中肠道失调的比较研究

Sarbjeet Niraula¹, Imadh Khan², Jonathan Jung³

¹Northwestern University.

Research square

|October 3, 2025

概括

便样本储存方法在患有外围动脉疾病 (PAD) 的患者中显著改变肠道微生物组的组成. 标准化储存对于准确的微生物组分析和心血管疾病研究中的疾病生物标志物发现至关重要.

科学领域:

微生物学微生物学
人类健康人类健康人类健康
基因组学就是基因组学.

背景情况:

大规模的肠道微生物组研究需要标准化的便样本存储.
之前关于储存效应的研究主要使用健康样本,不考虑疾病诱导的失生症.
心血管疾病,如周围动脉疾病 (PAD),与肠道失调有关,但存储效应尚不清楚.

研究的目的:

调查三个不同的便样本储存条件对PAD患者和健康对照患者肠道微生物群落的影响.
评估储存方法如何影响病态与非病态的微生物组成和复苏潜力.

主要方法:

一项涉及18名PAD患者和12名非PAD对照者的横截面研究.
便样本被立即冷 (IF),在修改的卡里-布莱尔 (CB) 或在OMNIgene•Gut瓶中储存.
进行了16S rRNA基因扩增序列测序 (V4区域);为复苏比较培养了甘油库存.

主要成果:

根据PAD和非PAD组的储存条件,观察到微生物组成和社区结构的显著差异.
与对照组相比,在PAD患者中发现了细菌属的存储条件特异性丰度差异.
与立即冷的样本相比,在卡里-布莱尔储存的样本显示活化潜力降低.

结论:

便样本的储存条件和持续时间显著影响肠道微生物群落和复苏潜力,特别是在PAD患者中.
存储方法的偏差可能会影响基于微生物组的疾病生物标志物发现.
在分析患病人群的便样本或组合来自不同队伍的数据时,标准化样本处理协议至关重要.

关键词:

生物标志物生物标志物失生症是一种失生症.胃肠道微生物组胃肠道微生物组健康的志愿者志愿者人类人类人类.微生物群中的微生物群周围动脉疾病周围动脉疾病

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