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相关概念视频

Hematopoiesis01:21

Hematopoiesis

8.7K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
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Overview of Hematopoiesis01:20

Overview of Hematopoiesis

8.3K
Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
8.3K
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

4.0K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
4.0K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.8K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.8K
Ribosome Profiling02:24

Ribosome Profiling

4.1K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.1K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

14.6K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
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相关实验视频

Updated: Jan 16, 2026

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
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Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

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RPS19和RPL5的哈普洛不充分模型揭示了胎儿血液形成的分离的核糖体子单元控制.

Lionel Blanc1, Yuefeng Tang2, Te Ling3

  • 1Feinstein Institute For Medical Research.

Research square
|October 3, 2025
PubMed
概括

钻石黑人贫症综合征 (DBAS) 是由核糖体蛋白 (RP) 缺血导致的. 新的模型揭示了胎儿血液发育中的明显的RP功能,不同影响了造血干细胞和红细胞生产.

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Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
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Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay

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Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
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Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

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相关实验视频

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Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
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Identification and Analysis of Mouse Erythroid Progenitors using the CD71/TER119 Flow-cytometric Assay
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Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
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科学领域:

  • 血液学 血液学 血液学
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 钻石黑贫血综合征 (DBAS) 是一种先天性核糖体病变.
  • 它是由核糖体蛋白质 (RP) 的哈普洛缺陷引起的.
  • 在红状腺发育中RP静脉测量和活性的确切作用尚不清楚.

研究的目的:

  • 为了研究小 (RPS19) 和大 (RPL5) 核糖体子单元蛋白在胎儿血液形成中的不同功能.
  • 阐明DBAS病原和临床异质性背后的机制.

主要方法:

  • 利用新的体内模型来研究RPS19和RPL5的哈普隆缺陷.
  • 分析了造血干细胞和原生细胞 (HSPC) 的动态.
  • 研究了p53介导的细胞死亡途径 (ferroptosis和apoptosis).
  • 评估了翻译和转录失调,包括RUNX1表达.

主要成果:

  • 由于RPL5的哈普洛缺陷导致HSPC的积累和产前死亡,通过红色素原体的ferroptosis.
  • 由于RPS19的哈普隆缺陷,导致HSPC的枯竭和通过亡导致红状腺扩张受损.
  • 由于RPS19缺乏,导致翻译/转录失调,包括RUNX1上调,反映了人类DBAS患者.
  • 在RPS19-haploins insufficient小鼠中,Runx1的删除部分挽救了HSPC的数量.

结论:

  • 来自不同核糖体子单元的RP在胎儿血液形成中起着不同的作用.
  • 不平衡的RP静态度会破坏发展计划,提供对DBAS的机械洞察力.
  • 这些发现解释了DBAS临床异质性的基础.