在猪†早期怀孕期间通过干 gamma 对子宫内膜免疫环境的编程
Mariana Sponchiado1, Weihong Gu1, Darling Melany de C Madrid1
1Division of Animal Sciences, University of Missouri, Columbia, MO, USA.
Biology of reproduction
|October 3, 2025
概括
概念衍生干扰素- (IFNG) 对于猪怀孕至关重要. 这项研究表明,IFNG调节了胚胎与母体接口的免疫细胞招募和巨细胞两极分化,影响了怀孕的成功.
科学领域:
- 生殖免疫学 生殖免疫学
- 哺乳动物胚胎学
- 怀孕的分子机制
背景情况:
- 来自早期猪概念的干扰素- (IFNG) 对于确定怀孕至关重要.
- 概念性IFNG在胚胎-母体界面和母体免疫细胞中的精确细胞和分子作用尚未完全理解.
研究的目的:
- 调查概念性衍生IFNG对母体免疫细胞基因表达和母体-母体接口的细胞群体在猪早期怀孕期间的影响.
- 阐明IFNG在怀孕期间影响免疫细胞功能和炎症的分子机制.
主要方法:
- 在外周血液单核细胞中分析了怀孕状态和IFNG目标基因 (IRF1) 表达.
- 使用CRISPR/Cas9基因编辑来创建缺少IFNG (IFNG-/-) 的概念体.
- 单核RNA测序和免疫组织化学被用来分析携带野生类型或IFNG-/-概念的子的子宫内膜组织.
主要成果:
- 在外围血液单核细胞中,怀孕对IRF1表达的上调.
- 单核RNA测序确定了细胞类型特异性的子宫内膜的转录组变化,以应对概念性IFNG.
- 在IFNG-/-样本中观察到单细胞和巨细胞的显著减少,IFNG与单细胞招募和M2巨细胞两极分化有关.
结论:
- 概念IFNG对于招募单细胞和促进胚胎-母体界面M2巨细胞两极分化至关重要.
- 这些IFNG介导的免疫细胞动态对于调节概念性附着过程中的炎症至关重要.
- IFNG激活的巨细胞的失调可能会导致猪的怀孕失败.
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