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碳化-可以作为在特定细胞系中体外基因毒性的阳性颗粒控制

Michael J Burgum¹, Stephen J Evans¹, Ilaria Zanoni²

¹In Vitro Toxicology Group, Faculty of Medicine, Health and Life Sciences, Institute of Life Sciences, Swansea University Medical School, Singleton Park, Swansea, Wales SA2 8PP, UK.

|October 3, 2025

概括

碳化- (WC/Co) 纳米颗粒显示出在体外基因毒性测试中作为积极对照的潜力. 他们在高度下诱导了TK6细胞中的微核和V79-4细胞中的突变,但在彗星测试中没有.

科学领域:

毒理学毒理学毒理学
纳米技术纳米技术
遗传学遗传学是一种遗传学.

背景情况:

在体外基因毒性测试需要可靠的阳性对照.
纳米粒子诱导的基因毒性机制尚未完全理解.
碳化- (WC/Co) 纳米粒子是积极控制材料的候选物.

研究的目的:

为了评估WC/Co纳米颗粒的基因毒性概况.
评估WC/Co作为基因毒性测试中的潜在阳性对照.
在人类淋巴细胞 (TK6) 和中国仓鼠肺纤维细胞 (V79-4) 中研究WC/Co的基因毒性.

主要方法:

细胞动力阻断微核 (CBMN) 试验.
哺乳动物细胞基因突变测试.
彗星测量. 彗星测量.
在TK6和V79-4细胞中24小时暴露.
传输电子显微镜用于细胞吸收.

主要成果:

在CBMN测定中,WC/Co纳米颗粒诱导了TK6细胞中的微核 (在100μg/ml时是2倍),而没有细胞毒性.
在使用CBMN试验的V79-4细胞中没有观察到基因毒性或细胞毒性.
在基因突变试验中,在V79-4细胞中观察到显著的突变性 (在100μg/ml时是2倍).
彗星测定在任何细胞类型中都没有显示出基因毒性.
通过传输电子显微镜没有检测到WC/Co的细胞吸收.

结论:

WC/Co纳米颗粒可以作为TK6细胞中CBMN试验的阳性对照.
WC/Co纳米颗粒可以作为V79-4细胞中基因突变试验的阳性对照.
作为阳性对照的有效使用需要测试的最高度 (100μg/ml).

关键词:

遗传毒性遗传毒性遗传毒性遗传毒性在体外 (in vitro) 的情况下.碳化的碳化的.

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