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相关概念视频

Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

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Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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相关实验视频

Updated: Jan 16, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

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看到红色,选择真:RUBY报告的种子标记器简化了CRISPR清洁大米育种.

Jin-Lei Liu1,2,3, Tao Yang1,3, Yu-Wei Fu4

  • 1State Key Laboratory of Forage Breeding-by-Design and Utilization, Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.

Rice (New York, N.Y.)
|October 3, 2025
PubMed
概括

我们为植物开发了一个新的CRISPR-Cas9工具包,使用视觉标记器轻松识别编辑后代. 这种方法简化了为研究创造基因稳定,无转基因的植物.

关键词:
克里斯普尔-Cas9是什么意思基因组编辑 基因组编辑红宝石 (Ruby) 是一种红宝石.没有转基因的自由基因.可见标记器辅助的选择

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Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
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Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
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Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

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相关实验视频

Last Updated: Jan 16, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
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Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
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科学领域:

  • 植物基因组学 植物基因组学
  • 基因编辑技术的技术
  • 分子生物学分子生物学

背景情况:

  • CRISPR-Cas9对于植物基因编辑至关重要,但对于稳定,转基因植物来说,删除编辑构造是必不可少的.
  • 目前用于删除CRISPR构造的方法通常是复杂的,并且不能普遍适用.

研究的目的:

  • 开发一种通用且操作简单的CRISPR-Cas9工具包,用于生成无转基因编辑植物.
  • 整合一个视觉标记器,以快速识别编辑的,无转基因的植物后代.

主要方法:

  • 开发了一个模块化的CRISPR工具包,其中包含视觉RUBY标记器.
  • 将工具包应用于大米 (cv. 钟华 11) 的CRISPR-Cas9基因组编辑.
  • 利用基因分离进行转基因去除和视觉查进行无转基因识别.

主要成果:

  • 在米中的三个独立的CRISPR-Cas9编辑事件中实现了100%的编辑效率.
  • 能够快速视觉识别无转基因后代.
  • 与传统方法相比,在识别编辑线条方面表现出显著的加速.

结论:

  • 开发的模块化CRISPR工具包与RUBY标记器提供了一个普遍适用的和操作简单的策略,用于生成无转基因编辑植物.
  • 该系统通过绕过艰苦的分子验证来加速编辑线的识别.
  • 该工具包提供了一个适应CRISPR载体在各种植物物种中进行基因组编辑的框架.