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脱基酶ZmUBP5对于玉米核的发展至关重要

Haixiao Dong¹, Li Zhang², Hao Chen¹

¹Jilin Province Engineering Research Center for Crop Biotechnology Breeding, College of Plant Science, Jilin University, Changchun, 130062, China.

The Plant journal : for cell and molecular biology

|October 3, 2025

概括

一种新的玉米突变揭示了二维基因酶ZmUBP5对核发育至关重要. 它与ZmEMB140相互作用,影响胚胎和内精子的大小以及植物活力.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

乌比基因化和杜比基因化是重要的翻译后修饰,调节了真核生物的生长和发育.
它们在玉米发展中的具体作用尚不清楚.

研究的目的:

为了研究在玉米核发育中的二维化酶的功能.
描述一种表现出发育缺陷的新型玉米突变.

主要方法:

乙基甲硫酸盐的突变发生,以产生突变物.
大量分离剂分析和CRISPR/Cas9用于基因识别.
亚细胞局部化,蛋白质相互作用和降解试验.

主要成果:

在ZmUBP5中停止获得的突变导致了较小的核,被捕的胚胎和色素缺陷.
ZmUBP5,一个核二维基因酶 (DUB),与ZmEMB140.0.互动.
ZmEMB140突变导致了较小的核,减少了胚胎/内精子的大小和致命的幼苗.

结论:

DUB ZmUBP5及其基质ZmEMB140对于玉米核的发育至关重要.
ZmUBP5通过duebiquitination调节核大小和胚胎/内精子的发育.
ZmEMB140涉及到mRNA前处理和结合体的功能.

关键词:

我们的EMB140是EMB140.UBP5 UBP5 UBP5 UBP5 UBP5 UBP5 UBP5 UBP5 UBP5 UBP5 UBP5 UBP5 齐亚梅斯 (Zea mays) 是一种植物.大量的分离分析 (BSA)必须进行脱离分离.核心突变的突变分子无处不在的化

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