RNAtranslator: 建模蛋白质条件RNA设计作为序列对序列自然语言翻译
Sobhan Shukueian Tabrizi1, Sina Barazandeh2, Helyasadat Hashemi Aghdam1
1Department of Computer Engineering, Bilkent University, Ankara, Türkiye.
PLoS computational biology
|October 3, 2025
概括
RNAtranslator是一种新的生成模型,通过将蛋白质信息转化为RNA序列来设计与蛋白质结合的RNA序列. 这推动了基于RNA的疗法和合成生物学,使各种蛋白质点的高效设计成为可能.
科学领域:
- 生物化学 生物化学
- 计算生物学 计算生物学
- 分子生物学分子生物学
背景情况:
- 蛋白质-RNA相互作用对于基因调节,拼接,RNA稳定性和翻译至关重要.
- RNA是一种有前途的治疗剂,可以向蛋白质,包括那些被认为是无毒的蛋白质.
- 设计用于选择性蛋白结合的RNA序列是具有挑战性的,原因是大量的序列空间和当前方法的局限性.
研究的目的:
- 开发一种新的计算方法来设计可选择性地与标蛋白结合的RNA序列.
- 克服传统体外选择和RNA设计的计算方法的局限性.
- 为了实现广泛的蛋白质的有效RNA设计,包括那些缺乏先前RNA相互作用数据的蛋白质.
主要方法:
- 介绍了RNAtranslator,一种生成语言模型,将蛋白质条件RNA设计作为一个序列对序列翻译问题.
- 在大规模数据集上训练模型,以学习RNA和蛋白质相互作用的联合表示.
- 直接生成给定蛋白质标的结合RNA序列,没有后代优化.
主要成果:
- RNA翻译器生成了具有自然性质和高新奇性的RNA序列.
- 与现有方法相比,设计的RNA序列表现出增强的结合亲和力.
- 该模型成功地为各种蛋白质点设计了RNA,包括那些没有可用的RNA相互作用数据的蛋白质点.
结论:
- RNAtranslator为设计具有特定蛋白质结合能力的RNA序列提供了一种高效和广泛适用的方法.
- 这种方法促进了基于RNA的新型治疗方法的开发,并促进了合成生物学应用.
- 生成型模型方法克服了RNA序列设计的关键挑战,扩大了向以前被认为是不可抗药的蛋白质的可能性.
相关概念视频
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Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
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