在因特林介导的内皮细胞对生物活性水凝的粘附中基于性别的差异的表征
Abbey Nkansah1, Aashee Budhwani1, Nicholas Grammer1
1Department of Biomedical Engineering, The University of Texas at Austin, Austin, Texas 78712, United States.
ACS biomaterials science & engineering
|October 3, 2025
概括
生物性别影响内皮细胞与生物材料的相互作用,影响器件性能. 了解整合素表达中的这些性别差异对于设计更好的接触血液的医疗设备至关重要.
科学领域:
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
- 心血管研究研究心血管研究
背景情况:
- 内皮质化是血液接触器件中血栓抵抗的关键.
- 当前的生物材料设计往往忽视了内皮细胞行为中的基于性别的差异.
- 综合素介导的细胞物质相互作用对内皮功能至关重要.
研究的目的:
- 研究人类冠状动脉内皮细胞 (HCEC) 与生物材料的相互作用中的性别差异.
- 阐明整合素-连接体相互作用在性别偏差内皮细胞反应中的作用.
- 为医疗器械的性别特定生物材料的设计提供信息.
主要方法:
- 从年龄相匹配的男性和女性捐赠者的HCEC中特征整合蛋白表达.
- 评估了HCEC粘附和扩散在聚乙烯糖醇 (PEG) 基水凝上与原蛋白或凝.
- 相关的整合素表达特征与观察到的细胞行为在不同的基质硬度.
主要成果:
- 在HCEC中确定了基于性别的独特的整合蛋白表达模式.
- 女性HCECs表现出α2β1,α5β1和αVβ3整合素的更高表达.
- 男性HCECs显示出更高的α1β1整合素表达.
- 与男性HCEC相比,女性HCEC在刚性水凝基板上表现出更大的附着性.
结论:
- 对生物活性水凝的内皮细胞反应的性别差异是由整合素相互作用介导的.
- 生物性别是一个重要的变量,在设计接触血液的生物材料时需要考虑.
- 根据性别量身定制生物材料可以增强血栓阻力和器件有效性.
相关概念视频
01:17Intracellular Signaling Affects Focal Adhesions
3.5K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
3.5K
01:15Activation of Integrins
4.9K
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
4.9K
01:10Integrins
5.2K
Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
5.2K