相关实验视频

Updated: Jan 16, 2026

Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans

Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans

Published on: February 13, 2013

通过RNAi介导的核受体HR96基因敲除抑制了Locusta migratoria的食和肠道发育

Xiaojian Liu¹, Juan Li¹, Suoli Zheng¹

¹Shanxi Key Laboratory of Nucleic Acid Biopesticides, Institute of Applied Biology, Shanxi University, Taiyuan, 030006, China.

International journal of biological macromolecules

|October 3, 2025

概括

我们在虫中发现了HR96基因,该基因对肠道发育和生存至关重要. 通过RNA干扰 (RNAi) 准该基因为虫害害管理提供了一个有前途的策略.

科学领域:

分子生物学分子生物学
昆虫学昆虫学是一门学科.
害虫管理害虫管理害虫管理

背景情况:

核受体 (NRs) 对昆虫的发育和生理学至关重要.
HR96在虫等半代谢昆虫中的功能在很大程度上是未知的.
了解虫的HR96可以揭示新的害虫控制目标.

研究的目的:

为了描述L. migratoria HR96 (LmHR96) 基因.
研究LmHR96在虫发育和生理学中的功能.
评估LmHR96作为基于RNAi的害虫控制的目标.

主要方法:

对LmHR96.96的基因鉴定和域分析.
用RT-qPCR分析LmHR96表达模式.
使用双链RNA (dsRNA) 向LmHR96.96的RNA干扰 (RNAi).
肠道组织的组织学和超结构分析.
用RNA测序来评估基因表达变化.

主要成果:

LmHR96具有特征性的NR DNA结合和联体结合域.
在 nymphal 阶段,LmHR96 在 integument 和肠道组织中高度表达.
LmHR96表达受到20E和LmEcR的调节.
通过RNAi介导的LmHR96沉默导致食停止,体重减轻,肠道细胞损伤和死亡率.
沉默LmHR96导致消化相关和信号通路基因的表达减少.

结论:

LmHR96对于L. migratoria的肠道形态发生和功能至关重要.
用RNAi针对LmHR96有效地抑制了虫的食和生存.
LmHR96代表了开发基于RNAi的虫防治策略的一个有希望的目标.

关键词:

HR96 HR96 是一个非常重要的数字.肠道肠道肠道肠道龙迁徙者 (英语:Locusta migratoria) 是一种迁徙的动物.核受体是一种核受体.这是一种RNA干扰.

更多相关视频

Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum

Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum

Published on: October 13, 2014

RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees Apis mellifera

RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees Apis mellifera

Published on: July 25, 2013

相关概念视频

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

Transducer Mechanism: Nuclear Receptors

Transducer Mechanism: Nuclear Receptors

Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes: