相关实验视频

Updated: Jan 16, 2026

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells

Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells

Published on: February 22, 2020

在LncRNARMRP中,Knockdown可以提高自然杀手细胞中PD-1表达的调节

Sarah Abouhegaziah¹, Doris Urlaub², Ahmed Moustafa^3,4

¹Microbiology, Immunology and Biotechnology Department, Faculty of Pharmacy and Biotechnology, The German University in Cairo, Cairo, Egypt.

Scientific reports

|October 3, 2025

概括

长非编码RNAs (lncRNAs) 在自然杀手 (NK) 细胞中被下调. 击败RMRP lncRNA会增加PD-1的表达,这表明免疫治疗的新目标.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

长非编码RNAs (lncRNAs) 调节基因表达而不编码蛋白质.
自然杀手 (NK) 细胞是具有细胞毒性功能的至关重要的先天性免疫细胞.
在NK细胞生物学中 lncRNAs 的特定作用在很大程度上仍未被探索.

研究的目的:

研究NK细胞中特定 lncRNAs 的功能作用.
分析NK细胞中五种选择的lncRNAs (MALAT1,RMRP,LINC-PINT,GAS5,LINC00299) 的表达模式.
探索NK细胞中 lncRNAs 和免疫检查点分子之间的关系.

主要方法:

在培养NK细胞中的lncRNAs的功能分析.
在基底和刺激条件下评估lncRNA表达水平.
研究RMRP淘汰对NK细胞PD-1受体表达的影响.

主要成果:

研究的五种lncRNA被发现在培养NK细胞中下调,并在细胞因子刺激后.
击败RMRP导致NK细胞表面PD-1受体的显著上调.
在NK细胞中提出了RMRP和PD-1表达之间的逆调节关系.

结论:

在NK细胞中调节PD-1表达时,RMRP可能起到抑制作用.
已识别的RMRP-PD-1轴为NK细胞免疫检查点调节提供了新的见解.
针对这种调节途径可能会导致新的免疫治疗策略来增强NK细胞功能.

关键词:

细胞因子刺激细胞因子刺激流动细胞计量流动细胞计量基因表达基因表达基因表达免疫检查站检查站免疫治疗是一种免疫疗法.长非编码RNA (lncRNA) 是一种长非编码RNA.自然杀手 (NK) 细胞在PD-1中使用PD-1.这是一个RMRP.

更多相关视频

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

Published on: January 9, 2018

相关概念视频

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...