相关实验视频

Updated: Jan 16, 2026

Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure

Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure

Published on: March 20, 2012

骨形态遗传蛋白与平面细胞极性通路之间的相互作用导致独特的乙醇诱导的面部缺陷

Raèden Gray¹, Anna Lloyd¹, C Ben Lovely¹

¹Department of Biochemistry and Molecular Genetics and Alcohol Research Center, School of Medicine, University of Louisville, Louisville, Kentucky, USA.

Alcohol, clinical & experimental research

|October 4, 2025

概括

胎儿酒精谱系障碍 (FASD) 与乙醇暴露有关. 这项研究表明,平面细胞极性 (PCP) 路径缺陷,而不是胃化,驱动斑马鱼面部发育中的乙醇敏感性.

科学领域:

发育生物学是发展生物学.
遗传学是一个遗传学.
甲状腺学甲状腺学

背景情况:

胎儿酒精谱系障碍 (FASD) 是由于在发育过程中暴露于乙醇造成的.
遗传因素影响乙醇易感性,但机制尚不清楚.
平面细胞极性 (PCP) 和骨形态遗传蛋白 (BMP) 信号通路对于胚胎发育至关重要,包括面部骨的形成.

研究的目的:

调查PCP突变体中乙醇敏感性的特定发育时间.
为了确定BMP信号是否与PCP通路相互作用,使其对乙醇敏感.
为了阐明PCP和BMP途径在面发育中的遗传相互作用.

主要方法:

在PCP和BMP信号通路发生突变的斑马鱼胚胎在各种发育窗口 (6-30hpf) 期间暴露在乙醇中.
用形态测量和线性测量来分析面部发育.
用基因分析来评估通路相互作用.

主要成果:

PCP突变体表现出显著的乙醇敏感性,主要是在受精后10至24小时之间,在胃化后.
BMP路径突变加剧了PCP突变者的乙醇敏感性,导致新的中线面缺陷.
PCP和BMP通路在内皮和神经 (CNC) 形态发生过程中发生基因相互作用.

结论:

乙醇诱导的面发育缺陷与胃流动后的PCP通路功能障碍有关.
PCP和BMP信号通路之间的相互作用对正常的面部发育至关重要,并影响乙醇敏感性.
这项研究有助于对乙醇诱导的出生缺陷的机制性理解,将细胞事件与发育结果联系起来.

关键词:

这就是 FASD 的原因.头骨面部的发展遗传学遗传学遗传学是一个斑马鱼是一种斑马鱼.

更多相关视频

Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage

Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage

Published on: October 21, 2015

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

Published on: January 30, 2014

相关概念视频

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...

Pleiotropy

Pleiotropy

Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...