同化压倒了盐水废水中的化 除:从异质化和有氧脱菌菌株到微生物群
Mengru Zhang1, Wenhao Zhang1, Qingyuan Jiang1
1School of Civil Engineering, Shandong University, 250061 Jinan, China; Laboratory of Water-Sediment Regulation and Eco-decontamination, 250061, Jinan, China.
Water research
|October 4, 2025
概括
使用型细菌的合成微生物组显示出盐水废水处理的前景. 这些工程社区将代谢从异质化和有氧脱化转向氨同化.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
- 废水处理 废水处理
背景情况:
- 异质化和有氧脱 (HN-AD) 是盐废水处理的一种新策略.
- 了解HN-AD在环境微生物组和代谢中的微生物应用至关重要.
研究的目的:
- 建立合成异质型微生物组使用性HN-AD菌株.
- 在纯培养菌株和人工微生物组中描述代谢.
- 研究生物膜形成菌株在微生物组合和循环中的作用.
主要方法:
- 使用生物膜形成的 Pseudomonas kunmingens 8-C 和 Acinetobacter johnsonii 2-1-H 菌株.
- 进行平衡,15N稳定同位素标记,酶活性测定和功能基因鉴定.
- 构建并分析了四种合成的性微生物组.
主要成果:
- 纯培养的HN-AD菌株通过同化和化去除了氨.
- 合成微生物组实现了76-92%的氨和72-86%的总去除.
- 形成生物膜的菌株促进了微生物组的决定性组合和选择性招募氨同化微生物 (由glnA基因丰度表明).
- 微生物组的代谢转向主导的氨同化.
结论:
- 使用功能性微生物构建环境微生物组的框架.
- 证明了从纯培养中的HN-AD转移到微生物联盟中的氨同化中代谢的转变.
- 强调了废水处理工程生态系统中生物膜形成和微生物相互作用的重要性.
更多相关视频
相关概念视频
01:22Inorganic Nitrogen Assimilation
459
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
459
01:15Metabolism of Chemolithotrophs
774
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
774
01:20Overview of Nitrogen Metabolism
11.0K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of ammonia, ammonium ions, nitrate, nitrite, or nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
11.0K
01:30Environmental Applications of Microorganisms
956
Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...
956
01:49The Nitrogen Cycle
59.4K
Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...
59.4K
00:46Bioremediation
22.0K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
22.0K