综合基质编辑和微流体提升了从素中产生微生物脂质的产生
Changyu Pi1, Jinyang Li1, Tongtong Bao1
1State Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
Bioresource technology
|October 4, 2025
概括
这项研究使用新基编辑器设计了Curvularia clavata J1真菌,以增强其处理性木质素 (AL) 的能力. 这种工程菌株显著增加了脂质生产和乳糖酶活性,为可持续的生物转化铺平了道路.
科学领域:
- 生物技术和合成生物学
- 微生物工程 微生物工程
- 可持续化学 可持续化学
背景情况:
- 氨酸是一种丰富的,可再生的芳香生物聚合物,具有可持续化学生产的潜力.
- 微生物有效地将红素转化为有价值的产品仍然是一个挑战,因为它的反抗性质.
研究的目的:
- 为了研究Curvularia clavata J1对性木质素 (AL) 的转录反应.
- 开发和应用一种新的全基因组基因编辑系统,用于改善真菌菌株.
- 为了增强素生物转化能力,增加脂质和酶的生产.
主要方法:
- 转录组分析以了解细胞对AL的反应.
- 开发一种可诱导铜的MCM5-AID基编辑器,用于向突变发生.
- 超高通量选使用滴滴微流体来识别改进的突变.
- 生物反应器培养以验证增强菌株性能.
主要成果:
- 在C. clavata J1中,AL诱导了电子运输,脂质代谢和铁稳态的上调调节.
- 工程突变M6的脂质含量增加了36% (干细胞重量增加了49%) 和乳糖酶活性增加了75% (228.58 U/L).
- 在生物反应器中验证的性能显示脂质增加33% (DCW的51%) 和乳糖酶活性增加87% (240.43 U/L).
- 基因组测序证实了C-to-T突变,转录组学揭示了增强的生物合成途径,将碳流重定向到富含油的单细胞蛋白.
结论:
- 这项研究提出了使用工程真菌进行宁生物转化的一体化框架.
- 合成生物学和微流体学的整合使精确的真菌工程能够提高生物产品的形成.
- 这种方法提供了一种可持续的战略,用于将木质素提升为有价值的生物化学品和生物燃料.
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