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miR-23a通过向Spodoptera frugiperda (史密斯) 中的SfGSTs3来调节兰利的敏感性

Fan-Bin Kong¹, Yong-Po Lv¹, Bai-Zhong Zhang²

¹School of Plant Protection and Environment, Henan engineering research center of biological pesticide & fertilizer development and synergistic application, Henan Institute of Science and Technology, Xinxiang, 453003, China.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP

|October 4, 2025

概括

秋季军对诸如兰利罗等杀虫剂的耐药性是一个日益严重的问题. 这项研究揭示了microRNA-23a调节了Glutathione S-transferases 3 (SfGSTs3) 的表达,影响了秋季军对杀虫剂的敏感性.

科学领域:

农业昆虫学农业昆虫学
分子生物学分子生物学
虫害管理虫害管理虫害管理

背景情况:

秋季军虫 (Spodoptera frugiperda) 在全球范围内造成了严重的作物损害.
对杀虫剂的耐药性,特别是对兰利的耐药性,是控制这种害虫的一个主要挑战.
谷氨S转移酶 (GSTs) 是关键酶,参与排毒和抗昆虫剂的抵抗.

研究的目的:

调查SfGSTs3在秋季军虫中兰利醇耐药性的作用.
探索SfGSTs3通过microRNA-23a.的转录后调节.
为了阐明微RNA介导的杀虫剂易感性的机制.

主要方法:

定量实时PCR分析SfGSTs3基因表达.
使用dsSfGSTs3评估其对杀虫剂敏感性的影响的RNA干扰 (RNAi).
路西法酶记者测试证实了miR-23a与SfGSTs3 3'UTR的结合.
在体内实验中使用miR-23a的agomir和antagomir注射.

主要成果:

在暴露于chlorantraniliprole的秋季军幼虫中,SfGSTs3的表达显著上调.
使用dsSfGSTs3沉默SfGSTs3增加了对兰利的敏感性.
microRNA-23a直接与SfGSTs3的3'UTR结合,调节其表达.
miR-23a agomir注射降低了SfGSTs3的表达,并增加了兰利的敏感性.
miR-23a对抗米尔注射增加了SfGSTs3的表达,并降低了兰利的敏感性.

结论:

在秋季军中,SfGSTs3在赋予兰利烯耐药性的过程中起着至关重要的作用.
微型RNA-23a作为SfGSTs3表达的负调节剂.
这项研究提供了对抗虫剂耐药性的分子机制的见解,并为害虫控制策略提供了潜在的目标.

关键词:

中的敏感性中的敏感性这是SfGSTs3s3 它们是花鸟 (Spodoptera frugiperda) 的种类.在 miR-23a 中.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...