沙门氏甲型杆菌通过CeRNA机制降低circHIPK2的表达,以抑制细胞迁移和瘤发生
Fei Wang1,2, Xiaoting Luan2, Huihui Ma2
1The Affiliated Mental Health Center of Jiangnan University, Wuxi Central Rehabilitation Hospital, Wuxi, 214151, Jiangsu, China.
Gut pathogens
|October 4, 2025
概括
在结肠直肠癌 (CRC) 中,沙门氏菌型菌感染会改变循环RNA (circRNA) 表达. 该circHIPK2/miR-124-3p通路是CRC病变发生的关键,也是潜在的治疗点.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 微生物学 微生物学
背景情况:
- 沙门氏菌是常见的肠道病原体.
- 循环RNAs (circRNAs) 在人类疾病的发病过程中至关重要.
- 主体circRNAs在S. typhimurium感染中在结直肠癌 (CRC) 发展过程中的作用尚不清楚.
研究的目的:
- 为了研究S. typhimurium感染的CRC中改变circRNA表达的治疗潜力.
- 阐明S. typhimurium在CRC中的circRNA调节的机制.
主要方法:
- 对感染了S. typhimurium. 的HCT116细胞进行转录组分析.
- circHIPK2的淘汰和过度表达的实验.
- 用RNA分子结合测试来确认相互作用.
主要成果:
- S. typhimurium 在HCT116细胞中显著改变了细胞circRNA表达.
- 循环HIPK2显著下调,其敲击抑制了细胞运动,瘤生长和细胞因子表达.
- circHIPK2与miR-124-3p相互作用,miR-124-3p的过度表达逆转了circHIPK2诱导的细胞运动效应.
结论:
- 受到S. typhimurium调节的circHIPK2/miR-124-3p轴在CRC病变发生过程中至关重要.
- 这一轴代表了结肠炎相关的CRC的一个有前途的分子治疗标.
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