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基于Sn/AuNP的高性能光谱圆测量传感器用于帕图林
Zafer Üstündağ1, Sinem Tunçer Çağlayan2, Mustafa Oguzhan Caglayan3
1Kütahya Dumlupınar University, Faculty of Arts and Science, Department of Chemistry, Kutahya, Türkiye.
Analytical biochemistry
|October 5, 2025
概括
一种新的无标签光谱圆测量感应传感器提供高度灵敏的帕图林检测. 这种方法实现了10 pg/mL以下的极限,用于在食品样本中准确分析真菌毒素.
科学领域:
- 生物感应是一种生物感应.
- 纳米技术纳米技术
- 分析化学 分析化学
背景情况:
- 帕图林 (PAT) 是一种在食物中发现的有害的真菌毒素.
- 准确而敏感的PAT检测方法对于食品安全至关重要.
- 现有的方法通常需要复杂的样本准备或标签.
研究的目的:
- 开发一种无标签的光谱圆测量 (SE) 适应传感器,用于超敏感的帕图林检测.
- 在灵敏度,动态范围和准确度方面调查apt传感器的性能.
- 为了验证适应传感器用于现实世界样本分析,例如果汁.
主要方法:
- (Sn) 基板与自组装单层通过电化学接种的制造.
- 用金纳米颗粒进行修改,并对抗PAT的aptamer进行固定.
- 通过监测相位变化,使用无标签的光谱圆测量检测帕图林.
- 使用循环电压测量和X射线光电谱学的表征.
主要成果:
- 在广泛的度范围内 (0.01-1000 ng/mL) 实现了对帕图林检测的线性反应.
- 经过证明的超敏感检测极限低至9.5 pg/mL.
- 在缓冲和刺果汁样品中表现出高精度 (%RSD <1.1%) 和准确性 (%RE在±5%以内).
- 结合动力学遵循伪二阶模型,表明有效的表面相互作用.
结论:
- 开发的无标签的SE口味传感器提供了一个敏感和可靠的平台来检测帕图林.
- 该方法需要最小的样本准备和没有外部标签,为例行分析提供了优势.
- 这种方法有望在食品工业中进行快速和准确的真菌毒素查.
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