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阿皮拉斯蛋白 (PsSP42) 在DNA结构中表达转染的COS-7细胞

Samira Hosseinpour Jahednia^1,2, Hossein Rezvan¹, Alireza Nourian¹

¹Department of Pathobiology, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran.

Iranian biomedical journal

|October 6, 2025

概括

这项研究证实了Leishmania PsSP42蛋白在COS-7细胞中使用两个DNA疫苗结构的成功表达. 这两种等离子体都表现出有效的蛋白质分泌,这对未来的疫苗开发至关重要.

科学领域:

分子生物学分子生物学
疫苗学疫苗学疫苗学
寄生虫学的寄生虫学

背景情况:

通过诱导幽默和细胞免疫,DNA疫苗显示出治疗细胞内寄生虫的潜力,例如Leishmania.
提供大型DNA疫苗构造的挑战可能会阻碍蛋白质表达和整体免疫疗效.
评估特定抗原的表达,如P. sergenti唾液蛋白 (PsSP42),是DNA疫苗开发的关键初步步骤.

研究的目的:

使用两个不同的等离子体载体,评估P. sergenti唾液蛋白 (PsSP42) 的体外表达.
为了比较一个小的,无抗生素的等离子体 (NTC9385R) 与一个常规的等离子体 (VR1020) 对PsSP42表达的疗效.
为了验证PsSP42的表达,在临床前对Leishmania的DNA疫苗研究中使用它之前.

主要方法:

使用两个等离子体进行了PsSP42的重组表达:NTC9385R和VR1020.
通过电穿孔和聚乙烯胺 (PEI) 介导的转染,COS-7细胞与等离子体进行了转染.
蛋白质表达得到了证实,使用来自 Ni-NTA 珠子的细胞超级生物的丰富后,西式涂抹.

主要成果:

两种VR1020-PsSP42和NTC-PsSP42结构都成功地在COS-7细胞中表达了PsSP42蛋白.
检测到表达的PsSP42蛋白,大约39.6kDa,在细胞超体中检测到,无论使用的转染方法.
在两种等离子体类型之间观察到类似的蛋白质表达水平在体外.

结论:

在NTC9385R和VR1020等离子体中,PsSP42蛋白在体外表达率相当.
传染方法 (PEI或电穿孔) 并没有显著影响PsSP42表达水平.
对DNA疫苗构造表达的临床前评估对于优化体内疗效至关重要.

关键词:

电穿孔是一种电子穿孔.基因表达基因表达基因表达热带鱼 (Leishmania tropica) 是一个热带鱼.聚乙烯胺的使用方法

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