在COPD呼吸道中SARS-CoV-2入口相关蛋白的表达:一种免疫组织化学研究
Larissa E Vlaming-van Eijk1, Zarlasht Sarsam1, Janna Bakker1
1Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
患有慢性阻塞性肺病 (COPD) 的患者表现出关键的SARS-CoV-2入口蛋白的呼吸道表达减少. 这可能会影响COVID-19的易感性,尽管确切的影响仍然不确定.
科学领域:
- 肺部医学 肺部医学
- 病毒学 病毒学
- 细胞生物学 细胞生物学
背景情况:
- 慢性阻塞性肺病 (COPD) 患者容易发生呼吸道感染和恶化.
- 在COPD患者中感染SARS-CoV-2的风险尚未完全理解.
- 了解病毒进入机制对于评估脆弱人群中COVID-19风险至关重要.
研究的目的:
- 为了比较COPD患者与非COPD对照者的呼吸道上皮质中SARS-CoV-2入口相关蛋白的表达.
- 为了研究蛋白质表达,COPD肺功能和对照组吸烟状态之间的关系.
主要方法:
- 27名COPD患者和40名非COPD对照患者的肺组织的免疫组织化学染色.
- 对HSPA5,NRP1,BSG,TMPRSS2,ITGB6,Furin,CTSL,ADAM17和ITGA5.5等呼吸道上皮表达蛋白的分析.
- 统计检查蛋白质表达,COPD肺功能和吸烟史之间的关联.
主要成果:
- 慢性肺炎患者在呼吸道上皮质中表现出HSPA5,NRP1,BSG,TMPRSS2和ITGB6的显著降低,这与吸烟无关.
- 两组之间没有发现Furin,CTSL,ADAM17和ITGA5表达的显著差异.
- 在COPD患者中,较低的BSG表达与肺功能减弱相关;在非COPD对照中,吸烟影响了几个蛋白质的表达.
结论:
- 慢性肺炎患者在他们的支气管表皮中显示了特定的SARS-CoV-2入口蛋白的减少表达.
- 对于COPD中COVID-19易感性的临床影响仍然不清楚,尽管病毒进入因素的潜在变化.
- 需要进一步的研究来阐明呼吸道因素,病毒入口和COPD中COVID-19结果之间的复杂相互作用.
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