在山中识别和分析expansin基因家族
Xing Qiao1,2,3, Shuai Yuan1,2,3, Jing Wei1,2,3
1College of Agriculture, Guangxi University, Nanning, Guangxi Zhuang Autonomous Region, China.
PeerJ
|October 6, 2025
概括
这项研究确定了子中的30个扩增基因 (EXP) 基因,揭示了它们在细胞壁松动和植物生长中的作用. 这些发现提供了对茎发展和扩张的见解.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 生物化学 生物化学
背景情况:
- 扩素是调节植物细胞壁扩展性的关键蛋白质,影响生长和发育.
- 缺乏对 *Dioscorea opposita* () 中的扩散基因家族的全面分析.
研究的目的:
- 为了识别和描述Dioscorea opposita*中的扩散 (DoEXP) 基因家族.
- 分析DoEXP基因的物理化学性质,系系和表达模式.
- 了解底层的分子机制 yam tuber 扩张和发展.
主要方法:
- 对公开可用的山基因组数据进行生物信息分析.
- 将DoEXP基因识别和分类为子家族.
- 对保存域,动机,外子-内子结构和促进区域的分析.
- 遗传学分析和对线性分析,以了解基因家族扩张.
- 用于功能注释的基因本体学丰富分析.
- 定量实时聚合酶连锁反应 (qPCR) 用于表达力验证.
主要成果:
- 在14个染色体中,30个DoEXP基因被确定并分为四个子家族 (DoEXPA,DoEXPB,DoEXLA,DoEXLB).
- 所有已识别的EXP蛋白共享保存的结构域 (DPBB_1和expansin_C) 和类似的动机/外因子-内子结构.
- 促进区含有与生长,植物激素信号和非生物压力相关的*cis*作用元素.
- 分段重复被确定为DoEXP基因家族扩张的主要驱动因素.
- GO分析将DoEXP基因与根延长和细胞分化联系起来.
- qPCR验证了9个选定的DoEXP基因的表达模式.
结论:
- 这项研究提供了对 *Dioscorea opposita* expansin 基因家族的全面描述.
- DoEXP基因在的生长,发育和茎扩张中起着重要的作用.
- 这项研究为了解茎发育的分子调节奠定了基础.
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