在过敏性鼻炎中,LncRNA PELATON通过miR-10b-5p/GATA3轴调节Th2细胞分化
Zhen Liu1,2, Yumei Li1,2, Xiangkun Zhao2,3
1Department of Otorhinolaryngology, Head and Neck Surgery, Yantai Yuhuangding Hospital, Qingdao University, Yantai 264000, China.
The World Allergy Organization journal
|October 6, 2025
概括
长非编码RNAPELATON通过调节miR-10b-5p/GATA3轴来促进过敏性鼻炎中的Th2细胞分化. 这种ceRNA网络为过敏性鼻炎提供了潜在的治疗点.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 长非编码RNAs (lncRNAs) 是免疫炎症疾病的关键.
- 在Th2驱动的过敏性鼻炎 (AR) 中 lncRNAs 的作用尚未完全理解.
- 这项研究重点关注lncRNA PELATON在AR病变发生中的作用.
研究的目的:
- 为了研究INcRNA PELATON在AR中的免疫调节功能.
- 探索PELATON对Th2细胞分化的调控作用.
- 为了阐明AR中PELATON的机制.
主要方法:
- 从16名AR患者和16名对照组收集了样本.
- 在使用qPCR的PBMC中量化PELATON表达.
- 在PELATON/miR-10b-5p操纵后评估Th2细胞分化和细胞因子水平 (GATA3,IL-4,IL-5,IL-13).
- 利用转录组测序和双露西法酶记者测试来确认相互作用.
主要成果:
- 皮拉顿表达在AR患者中较高,与过敏严重程度相关.
- 皮拉顿的过度表达增加了Th2细胞比例和关键细胞因子水平.
- 皮拉直接与miR-10b-5p结合,抑制其抑制GATA3.3的发生.
- 确定了一个促进Th2分化的ceRNA网络 (PELATON/miR-10b-5p/GATA3).
结论:
- LncRNA PELATON通过PELATON/miR-10b-5p/GATA3 ceRNA网络促进了Th2细胞的分化和AR中的激活.
- 这种监管机制为AR提出了新的治疗目标.
- 皮拉顿是AR严重程度的潜在生物标志物.
更多相关视频
12:21Examining the Role of Nasopharyngeal-associated Lymphoreticular Tissue NALT in Mouse Responses to Vaccines
Published on: August 1, 2012
27.4K
10:39Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy
Published on: April 16, 2019
8.1K
相关概念视频
02:39lncRNA - Long Non-coding RNAs
9.8K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.8K
02:39lncRNA - Long Non-coding RNAs
3.5K
3.5K
01:24T Cell Types and Functions
2.1K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
2.1K
01:16TGF - β Signaling Pathway
10.5K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.5K