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通过减少普鲁士蓝薄膜检测细菌活动,由拉曼光谱检测

Bruna F Baggio^1,2, Nga Tsing Tang¹, Cristiano Vicente²

¹Open Innovation Hub for Antimicrobial Surfaces, Surface Science Research Centre, Department of Chemistry, University of Liverpool, Liverpool L69 3BX, United Kingdom.

|October 6, 2025

概括

检测微生物和生物膜是一项挑战. 普鲁士蓝 (PB) 薄膜通过细菌代谢转化为普鲁士白 (PW),可以通过拉曼光谱检测,从而实现敏感的局部微生物传感.

科学领域:

材料科学材料科学材料科学
生物技术是生物技术.
分析化学分析化学

背景情况:

在各种科学领域,无需生物标记物即位检测微生物和生物膜仍然是一个重大挑战.
普鲁士蓝 (PB) 是一种多功能材料,在药物输送,毒药解毒剂,光热疗法和活死细菌检测方面具有证明的应用.

研究的目的:

为了研究电沉积的普鲁士蓝 (PB) 薄膜用于检测细菌活动的能力.
通过使用PB膜和拉曼显微光谱学来监测 * Staphylococcus aureus * 生物膜的代谢活性.

主要方法:

普鲁士蓝 (PB) 薄膜的电沉积.
化PB薄膜与*黄金葡萄球菌* (10^7 CFU mL^-1) 形成生物膜.
使用拉曼显微光谱学追踪PB转化为普鲁士白 (PW).
使用局部拉曼映射来观察PB减少的空间变化.

主要成果:

拉曼光谱检测到PB膜减少的开始大约2.5小时后化与黄金菌.
局部拉曼映射显示,PB减少的程度与细菌生物膜的存在和代谢活性相关.
通过*S. aureus*代谢活动将PB转化为PW,成功地以时间和空间分辨的方式观察到.

结论:

通过拉曼光谱检测细菌活动减少PB膜,为感知活体,代谢活跃的微生物提供了一种敏感,直接和非破坏性的方法.
这种方法可以局部检测微生物的存在和活动,解决生物膜监测的一个关键挑战.
电子沉积的PB薄膜显示为实时,现场微生物检测系统的基板具有前途.

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