探索咖啡因提取使用疏水性深度修养溶剂:实验和理论方法.
Khatereh A Pishro1, Leandro S Silva1, Rafaela S Lamarca1
1Department of Chemistry and Environmental Science, São Paulo State University (UNESP), São José do Rio Preto, São Paulo 15054-000, Brazil.
ACS omega
|October 6, 2025
概括
疏水性深度浸泡溶剂 (HDESs) 为从咖啡豆和其他来源提取咖啡因提供了一个可持续的替代方案. 与传统溶剂相比,dl-mentol/酸HDES的效率和绿色度都更高.
科学领域:
- 绿色化学和可持续的提取技术
- 分析化学和光谱光度学.
- 溶剂科学与工程 溶剂科学与工程
背景情况:
- 传统的有机溶剂在液体液体提取过程中对环境和健康构成风险.
- 由于其生物降解性和低毒性,疏水型深溶剂 (HDES) 正在成为环保的替代品.
- 像咖啡因这样的生物活性化合物的有效提取对各种行业至关重要.
研究的目的:
- 调查特定的HDESs (用乙酸或六酸的dl-menthol) 对于咖啡因提取的疗效.
- 优化提取条件并评估HDES与传统溶剂的性能.
- 评估开发的基于HDES的提取方法的可持续性和绿色性.
主要方法:
- 使用COSMO-RS建模进行溶剂选,以确定有前途的HDES系统.
- 从咖啡豆,咖啡皮和瓜拉纳饮料中提取咖啡因,使用精选的HDES.
- 通过UV/vis光谱光度测量与高斯适配以尽量减少光谱干扰来量化提取的咖啡因.
- 确定最佳提取参数 (温度,溶剂比) 和方法验证 (精度,LOD,LOQ).
- 使用分析绿色度 (AGREE) 指标对方法可持续性的评估.
主要成果:
- 与dl-mentol/hexanoic acid (0.610 mg/100 mg) 相比,dl-mentol/acetic acid HDES实现了更高的咖啡因提取产量 (0.765 mg/100 mg来自咖啡豆) 和dl-mentol/hexanoic acid (0.610 mg/100 mg) 相比.
- 最佳的提取条件是在65°C,溶液与溶剂的比例为1:1.
- 该方法显示了高精度 (SD ±0.0826毫克L-1) 和低检测/定量限值 (分别为0.674和2.04毫克L-1).
- 该HDES方法获得了0.83的高AGREE分数,明显优于二甲和等传统溶剂.
结论:
- HDES,特别是dl-mentol/酸,是提取咖啡因的有效和可持续的溶剂.
- 开发的方法与绿色化学原则保持一致,为传统提取技术提供更安全,更有效的替代方案.
- 理论建模 (COSMO-RS) 和实验验证都支持HDES在绿色提取过程中的潜力.
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