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通过阻行测量免疫传感器敏感检测固土壤细菌

Napolean Bonaparte Thanapaul^1,2, Shrijana Bista^1,2, Panlada Tittabutr²

¹Molecular Biotechnology Laboratory, Suranaree University of Technology, Suranaree, Muang, Nakhon Ratchasima 30000, Thailand.

|October 6, 2025

概括

一个新的抗体生物传感器可以快速检测到Bradyrhizobium sp. SUTN9-2,一个关键的固细菌,用于可持续农业. 这项创新能够有效地监测土壤中的生物肥料含量,以提高作物产量.

科学领域:

农业微生物学农业微生物学
生物技术是生物技术.
生物传感器技术技术

背景情况:

菌细菌对于豆类植物的固定至关重要,减少了对合成肥料的依赖.
布拉迪里索比乌姆 (Bradyrhizobium sp.) 是一种植物. SUTN9-2是豆类-大米系统的强有力的生物肥料,增强了植物生长和耐压力.
准确和快速地对生物肥料菌株进行土壤监测对于可持续农业至关重要.

研究的目的:

开发和优化一种基于抗体的生物传感器,用于检测Bradyrhizobium sp. 在SUTN9-2.
为了使土壤中的生物肥料度能够快速,具有成本效益和便携式监测.

主要方法:

通过通过DSP自组装单层将特定的重组抗体固定在金电极上来制造免疫传感器.
检测到Bradyrhizobium sp. 的情况使用电化学阻抗光谱 (EIS) 的SUTN9-2.
优化和验证生物传感器的性能,包括特异性和检测范围.

主要成果:

开发的免疫传感器对Bradyrhizobium sp.具有很高的特异性. 在SUTN9-2.
实现了从1 x 10^3到1 x 10^7 CFU/mL (R^2 = 0.992) 的广泛线性检测范围.
传感器显示了接近单细胞水平的检测极限和与土壤样本的兼容性.

结论:

基于抗体的生物传感器为便携式和高效的Bradyrhizobium sp.监测提供了一个有前途的平台. 在土壤中的SUTN9-2.
这项技术可以支持在可持续农业中部署生物肥料.
该平台可适应检测其他有益的微生物菌株.

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