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相关概念视频

Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Updated: Jan 16, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
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基于散射的紧型光板显微镜探针使用定制的微型客观镜头.

Jingwei Zhao1, Yongjun Kim2, Momoka Sugimura1

  • 1University of Arizona, James C. Wyant College of Optical Sciences, Tucson, Arizona, USA, 85721.

Journal of optical microsystems
|October 6, 2025
PubMed
概括
此摘要是机器生成的。

一个新的紧的分散式光板显微镜 (sLSM) 探测器以细胞分辨率对未染色的组织进行图像. 这种创新的显微镜工具为生物成像提供了高分辨率和宽视野.

关键词:
紧型显微镜 紧型显微镜定制的客观镜头镜头客观镜头这是一种内在的散射.光片显微镜光片显微镜微型客观镜头的小镜头.没有染色的组织成像.

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科学领域:

  • 生物医学工程 生物医学工程
  • 光学显微镜的使用方法
  • 细胞成像 细胞成像

背景情况:

  • 光板显微镜 (LSM) 能够对生物样本进行高分辨率成像.
  • 现有的LSM系统可能很庞大,需要专门的设置.
  • 绘制未染色的组织图像在实现细胞分辨率方面存在挑战.

研究的目的:

  • 为了开发一个基于散射的紧型光板显微镜 (sLSM) 探针.
  • 为了实现未染色组织的细胞分辨率成像.
  • 创建一个便携式和高性能显微镜解决方案.

主要方法:

  • 开发一个定制的微型镜头镜头,提供高侧分辨率和大视野 (FOV).
  • 组件集成到一个紧的探头 (4cm x 4cm x 10cm).
  • 探测器轴向和侧向分辨率和成像深度的表征.

主要成果:

  • 定制镜头通过± 1.0mm的FOV实现了1.65-1.97μm的横向分辨率.
  • 紧的sLSM探头在206.2μm深度上显示了一个轴分辨率<5.6μm.
  • 固定的人类上皮质组织中的细胞细节被可视化,可与基板LSM相比.

结论:

  • 一个能够对未染色的组织进行高分辨率成像的紧型sLSM探针已经成功开发出来.
  • 该探测器在生物研究的便携性和性能方面提供了显著的优势.
  • 这项技术在需要详细组织可视化的各个领域都有潜在的应用.