基于散射的紧型光板显微镜探针使用定制的微型客观镜头
Jingwei Zhao1, Yongjun Kim2, Momoka Sugimura1
1University of Arizona, James C. Wyant College of Optical Sciences, Tucson, Arizona, USA, 85721.
概括
一个新的紧的分散式光板显微镜 (sLSM) 探测器以细胞分辨率对未染色的组织进行图像. 这种创新的显微镜工具为生物成像提供了高分辨率和宽视野.
科学领域:
- 生物医学工程 生物医学工程
- 光学显微镜的使用方法
- 细胞成像 细胞成像
背景情况:
- 光板显微镜 (LSM) 能够对生物样本进行高分辨率成像.
- 现有的LSM系统可能很庞大,需要专门的设置.
- 绘制未染色的组织图像在实现细胞分辨率方面存在挑战.
研究的目的:
- 为了开发一个基于散射的紧型光板显微镜 (sLSM) 探针.
- 为了实现未染色组织的细胞分辨率成像.
- 创建一个便携式和高性能显微镜解决方案.
主要方法:
- 开发一个定制的微型镜头镜头,提供高侧分辨率和大视野 (FOV).
- 组件集成到一个紧的探头 (4cm x 4cm x 10cm).
- 探测器轴向和侧向分辨率和成像深度的表征.
主要成果:
- 定制镜头通过± 1.0mm的FOV实现了1.65-1.97μm的横向分辨率.
- 紧的sLSM探头在206.2μm深度上显示了一个轴分辨率<5.6μm.
- 固定的人类上皮质组织中的细胞细节被可视化,可与基板LSM相比.
结论:
- 一个能够对未染色的组织进行高分辨率成像的紧型sLSM探针已经成功开发出来.
- 该探测器在生物研究的便携性和性能方面提供了显著的优势.
- 这项技术在需要详细组织可视化的各个领域都有潜在的应用.
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