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基于酶的系统,用于尼古丁胺胺辅因子之间的转
Haizhao Xue1,2, Yinghan Hu1,2, Aabid Manzoor Shah1
1Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Synthetic and systems biotechnology
|October 6, 2025
概括
这项研究引入了一种新的酶介导方法,用于将尼古丁胺氨基二核酸 (NAD) 和尼古丁胺酸二核酸 (NCD) 辅因子之间的降解等价物转移. 这种方法促进了辅因子相互转换,为代谢工程提供了一个新的工具.
科学领域:
- 生物化学 生物化学
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 尼古丁胺胺共因子如NAD和NADP对于细胞氧化还原反应至关重要.
- 一种非自然的辅助因子,尼古丁胺胺细胞因子二核酸 (NCD),扩大了辅助因子谱.
- 在辅因子之间有效地转移减少等价物仍然是代谢工程的一个挑战.
研究的目的:
- 开发一种新的策略,使不同辅助因子之间的等价交换减少.
- 为了利用酶 (ME) 介导的过化进行辅因子相互转换.
- 为了证明这种方法的可行性,在体外和体内.
主要方法:
- 采用野生类型和人工酶 (ME和ME*),有利于不同的辅因子 (NAD,NADP,NCD).
- 建立了一个体外系统,使用NADH,NCD,ME,ME*和pyruvate来评估辅因子转化.
- 在非传染性疾病自给的大肠杆菌细胞中实施了该系统,以观察减少等效流量.
主要成果:
- 在体外实验表明,在2小时内,NADH消耗高达65%,NCDH生成高达57%.
- 在大肠杆菌中,该系统成功地将降解等价物导向了与NCDH相关的乳酸盐形成.
- 使用ME介导的跨化证明了辅因子相互转换.
结论:
- 开发了一种有效的策略,通过辅助因子 transhydrogenation 调节细胞内还原剂等价物.
- 介于ME的方法为代谢工程和合成生物学应用提供了一个新的工具.
- 促进了减少等值的合理转移,克服了一个长期存在的挑战.
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