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对2型糖尿病和败血症之间共有的干细胞相关基因的综合分析

Yubo Wang¹, Xinhai Jiang², Xiaohan Song³

¹Department of Pharmacy, Beijing Ditan Hospital, Capital Medical University, Beijing, China.

Frontiers in chemistry

|October 6, 2025

概括

研究人员确定了两个关键的干细胞相关基因,CAPG和DDAH2,作为2型糖尿病 (T2DM) 和败血症的常见生物标志物. 这些发现为治疗这些相互关联的疾病提供了新的分子见解.

科学领域:

基因组学和生物信息学
免疫学免疫学免疫学
内分泌学在内分泌学.

背景情况:

2型糖尿病 (T2DM) 损害免疫功能,导致胰岛素抵抗,增加感染和败血症的易感性.
败血症会加剧糖尿病并发症,造成一个有害的循环.
基于干细胞的疗法对T2DM和败血症都很有前途,这表明共享的潜在机制.

研究的目的:

为了确定T2DM和败血症之间共享的常见干细胞相关基因 (SCRGs).
发现T2DM和败血症的新型干细胞相关生物标志物.
阐明潜在的分子通路和细胞机制,将T2DM与败血症联系起来.

主要方法:

利用基因表达综合 (GEO) 数据集 (GSE15932用于T2DM,GSE65682用于败血症) 来识别常见的差异表达基因 (DEG).
交叉DEG与SCRG以确定共享的差异表达SCRG (DE-SCRG).
使用功能相似性分析,机器学习,ROC曲线,功能丰富分析,免疫透分析和单细胞分析来验证生物标志物和探索途径,通过体外T2DM-败血症模型得到证实.

主要成果:

在T2DM和败血症中确定了CAPG和DDAH2作为明显上调的常见DE-SCRG.
功能性丰富涉及CAPG和DDAH2在"FC马R介导的细胞分裂"和"核糖体"途径.
在疾病和对照组之间观察到不同的免疫细胞透模式 (CD8 T细胞,单细胞) 和胰腺细胞 (α,β,delta,PSCs) 中的差异性CAPG表达,在体外T2DM-败血症模型中验证了转录和蛋白质水平.

结论:

发现CAPG和DDAH2作为T2DM和败血症的新型共享干细胞相关生物标志物.
阐明了涉及细胞和核糖体的常见途径,可能将这些条件联系起来.
为未来研究T2DM和败血症的分子机制和治疗策略提供了基础的见解.

关键词:

生物标志物生物标志物机器学习是机器学习.这是一种血症.干细胞是干细胞的组成部分.2型糖尿病是什么?

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