从涂抹到测序:使用甲状腺细胞学涂抹幻灯片进行ThyroSeq分子分析和来自61个测试结节的临床病理见解
Levent Trabzonlu1,2, Caroline Elliott1,3, Adam S Fisch1
1From the Department of Pathology, Massachusetts General Hospital, Boston (Trabzonlu, Elliott, Fisch, Chebib, Madrigal).
Archives of pathology & laboratory medicine
|October 6, 2025
概括
在没有标准样本的情况下,可以使用甲状腺细胞学涂抹片进行分子测试,提高诊断产量并减少不确定的结节的重复程序. 这种方法确保了及时的分子分析和患者护理.
科学领域:
- 内分泌学 在内分泌学.
- 分子病理学分子病理学
- 细胞病理学 细胞病理学
背景情况:
- 甲状腺结节不确定细胞学结果往往需要分子测试来准确诊断.
- 传统的测试介质可能并不总是可用,这给分子分析带来了挑战.
研究的目的:
- 提出一种方法来克服使用甲状腺细胞学涂抹幻灯片的ThyroSeq测试中的挑战.
- 报告使用涂抹片对甲状腺结节的分子分析结果.
主要方法:
- 开发软件来解析非结构化的ThyroSeq报告以提取数据.
- 数字化涂抹片以创建替代样本记录用于分子分析.
- 简化了测试发送过程,并记录了临床,分子和病理学发现.
主要成果:
- 使用ThyroSeq.测试了61个甲状腺细针吸收标本.
- 在8个案例中,涂抹片是分子测试的唯一可行的样本.
- 21个标本 (34.4%) 显示ThyroSeq阳性结果,包括基因突变,拷贝数改变和基因融合.
结论:
- 甲状腺细胞学涂抹片可以有效地用于分子分析,特别是当常规样本不足时.
- 高的充分率和分子变化的成功检测表明了涂抹片在分子测试中的潜力.
- 这种方法减少了重复手术的需要,并简化了患者的护理,尽管有效的团队沟通至关重要.
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