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相关实验视频

Updated: Jan 15, 2026

Sample Preparation for Mass Cytometry Analysis
06:28

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

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基于DLD的芯片样本处理用于无标签单细胞MS细胞计.

Junwen Zhu1, Yingqi Yang1, Chenxi Cao1

  • 1State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instrument, Tsinghua University, Beijing 100084, P. R. China. wwh@tsinghua.edu.cn.

Analytical methods : advancing methods and applications
|October 6, 2025
PubMed
概括

这项研究引入了一种新的微流体芯片,用于无标签质量细胞计. 它使用确定性横向位移高效地分类和准备单个细胞进行分析,从而实现样本输入结果输出质谱.

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科学领域:

  • 生物化学 生物化学
  • 分析化学 分析化学
  • 微流体学 微流体学

背景情况:

  • 单细胞质谱 (MS) 对于生物化学分析至关重要.
  • 无标签的质量细胞计将MS与微流体相结合.
  • 在芯片上有效地处理细胞样本至关重要,但在MS细胞计量方面尚未得到充分发展.

研究的目的:

  • 开发一种被动微流体方法,用于单细胞多发性硬化分析的细胞样本处理 (分类和淡化).
  • 为了整合确定性横向位移 (DLD) 进行液态动力学细胞操纵.
  • 为连续多发性硬化细胞计量创建一个样本输入结果输出系统.

主要方法:

  • 利用两个连续的DLD数组来按大小对细胞进行聚焦并对目标细胞进行排序.
  • 用MS提取溶液替换的细胞溶液在芯片上.
  • 集成了一个电子喷射电离毛细管与芯片上的液态金属合金电极用于MS分析.

主要成果:

  • 成功演示了一种使用DLD的被动细胞样本处理系统.
  • 通过样本输入结果输出工作流程实现了连续的MS细胞计量分析.
  • 收集了令人满意的MS细胞测量数据,以区分三种细胞类型.

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结论:

  • 拟议的基于DLD的微流体芯片为MS分析中单细胞样本处理提供了一种有效的方法.
  • 这项技术可以作为一个独立的模块或集成组件,用于芯片上MS细胞计量.
  • 它为单细胞成员国的有效样本准备建立了新的范式.