相关实验视频
Updated: Jan 15, 2026

13:19
Enhanced Oil Recovery using a Combination of Biosurfactants
Published on: June 3, 2022
5.9K
由Bacillus subtilis群体进行具有抗微生物活性的酶和生物表面活性剂生产
Mariana Prósperi de Oliveira Paula1, Clara Resende de Souza Castro1, Juliete Gomes de Lara1
1Agricultural Microbiology, Department of Biology, Federal University of Lavras (UFLA), Lavras, 37200-900, MG, Brazil.
Archives of microbiology
|October 6, 2025
概括
细菌细菌菌株从废物甘油中产生生物表面活性剂,具有抗微生物和乳化特性. 这些微生物代谢物为生物控制和生物修复应用提供了潜力.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 环境科学 环境科学
背景情况:
- 生物活性剂是微生物产品,具有多种工业和环境用途.
- 来自生物柴油生产的剩余糖是一种潜在的低成本基质,用于微生物代谢物合成.
研究的目的:
- 通过使用残留糖醇,研究Bacillus subtilis组菌株的生物表面活性剂生产.
- 评估产生的生物表面活性剂的酶和抗微生物活性.
- 评估生物控制和生物修复的潜在应用.
主要方法:
- 利用PCR检测表面因子,基因和iturin生物合成的基因.
- 培养的Bacillus subtilis菌株使用原始甘油作为唯一的碳来源.
- 测量了柴油乳化,降低表面张力,抗微生物活性和化酶活性 (MnP,LiP).
主要成果:
- 在Bacillus subtilis菌株中确认了生物表面活性剂生产基因.
- 已证明,原始糖支持生物表面活性剂的合成,乳化率高达61%,表面张力降低到30mN/m.
- 展示了对大肠杆菌和Fusarium paranaense的抗菌活性,以及显著的过氧化酶 (MnP) 和过氧化酶 (LiP) 活性.
结论:
- 细菌菌群菌株有效地从废物甘油中产生生物表面活性剂.
- 生物表面活性剂和相关酶具有生物控制和生物修复的潜力.
- 鉴定出Bacillus subtilis CCMA 2031具有针对植物病原体Fusarium paranaense的活性.
相关概念视频
Biological Methods for Microbial Control
783
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
783
Chemical Agents for Microbial Control
759
Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
759
Surface Membrane Barriers
2.6K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
2.6K
Microorganisms in Agriculture and Food industry
1.3K
Microorganisms play a crucial role in agriculture and the food industry, contributing to soil fertility, crop protection, and food production. Their functions range from nitrogen fixation and biopesticide production to fermentation and food preservation, making them indispensable to sustainable farming and food safety.Role in AgricultureNitrogen-fixing bacteria, such as Rhizobium (symbiotic) and Azotobacter (free-living), convert atmospheric nitrogen into ammonia through biological nitrogen...
1.3K
Gene Regulation in Microbial Communities: Quorum Sensing
518
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
518
Biofilms
1.1K
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
1.1K

