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单细胞质谱学代谢学研究的样本准备:结合细胞洗,杀,干燥和储存

Deepti Bhusal1, Shakya Wije Munige1, Zongkai Peng1

  • 1Department of Chemistry and Biochemistry, University of Oklahoma.

Journal of visualized experiments : JoVE
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PubMed
概括

一个新的协议保留了单细胞质谱 (SCMS) 分析的细胞代谢物完整性. 这种方法使用快速火和冷干燥来保持活细胞中的代谢物概况,以获得准确的代谢学.

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Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...