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Updated: May 10, 2026

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
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Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

Published on: June 7, 2018

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热诱导的蛋白质氧化作为热稳定性测量障碍:关于细胞染色体c的案例研究

Evelyn H MacKay-Barr1, Lars Konermann1

  • 1Department of Chemistry, The University of Western Ontario, London, Ontario N6A 5B7, Canada.

Analytical chemistry
|October 6, 2025
PubMed
概括

蛋白质稳定性的热展开实验通常是不可逆转的,因为氧化变化. 质谱检测显示了Tyr,Trp和Met残留的氧化,质疑了热力学参数的有效性. 对于氧化抑制策略需要进一步研究.

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Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

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相关概念视频

Protein Denaturation01:28

Protein Denaturation

The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...