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由CRISPR-Cas12a系统驱动的新型电化学发光共振能量传输生物传感器用于ctDNA检测

Xiaocui Huang¹, Alian Wang¹, Zhenyu Lin²

¹Department of Science Research and Training, Fujian Institute of Education, Fuzhou, 350001, China.

Biosensors & bioelectronics

|October 6, 2025

概括

这项研究介绍了一种新的电化学发光生物传感器,用于检测循环瘤DNA (ctDNA). 该系统利用CRISPR-Cas12a和共振能量转移来检测肺癌中敏感的L858R突变.

科学领域:

生物医学工程生物医学工程
生物感知技术的技术
分子诊断学分子诊断学

背景情况:

对电化学发光 (ECL) 生物传感器来说,共振能量转移 (RET) 是至关重要的.
控制能量捐赠器-接受器距离是高效ECL-RET系统的关键.
开发用于循环瘤DNA (ctDNA) 的敏感和特定的生物传感器对于癌症诊断至关重要.

研究的目的:

开发一种新的ECL-RET生物传感器,用于同质检测ctDNA.
为了提高灵敏度,将CRISPR-Cas12a系统与ECL-RET机制集成在一起.
检测非小细胞肺癌患者ctDNA中的特定突变,如L858R.

主要方法:

使用嵌入在DNA和Au纳米 (能量接受器) 中的Ru(phen) 3^2+ (能量捐赠者) 构建了一个新的ECL-RET系统.
开发了一种均的ECL生物传感器,利用目标激活的CRISPR-Cas12a系统来驱动ECL-RET效应.
应用生物传感器检测ctDNA并识别非小细胞肺癌患者的L858R突变.

主要成果:

该ECL生物传感器证明有效地识别了ctDNA中的L858R突变.
实现了对ctDNA的广泛线性检测范围,从10 fM到1 nM.
获得了ctDNA的极好的低检测极限3.0 fM.

结论:

开发的ECL-RET生物传感器为ctDNA检测提供了一种高效和特定的方法.
CRISPR-Cas12a和ECL-RET的组合显著提高了生物传感器的灵敏度.
这种方法对早期诊断和监测非小细胞肺癌有希望.

关键词:

在CRISPR-Cas12a系统中.循环瘤DNA是循环中的瘤DNA.电化学发光共振振荡能量转移能量转移.一致的传感感应.在L858R突变中.

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